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Organic Framework@Coordination Polymer Core-Shell Composites as Dual-Modal probe for Fluorescence and Colorimetric Analysis of Total Antioxidant Level in Saliva

2021; Elsevier BV; Volume: 347; Linguagem: Inglês

10.1016/j.snb.2021.130588

1873-3077

Wei Xue, Jian Chen, Xinyu Zhang, Ziqing Zhu, Huili Liu, Xinsheng Wang, Xuming Guo, Baocheng Yang,

Luminescence and Fluorescent Materials

The total antioxidant level is an important biomarker to determine redox imbalance in human body which can cause many diseases and threaten human health. Though the detection of antioxidants in vivo is gaining wide attention, most of the developed methods have only one detection mode and can only detect single type of antioxidant, leading to inaccurate test results. Here, we developed [email protected](III)-ART NPs as a dual-modal probe for water soluble total antioxidants detection in saliva. The detection mechanism was based on two reactions, including a cascading reaction of antioxidants activating the production of free radicals for fluorescence components generated, and a coordination reaction of antioxidants capturing Cu2+ from the MOFs nodes for carboxyl group protonated. Based on this, the UV–vis absorbance intensity and fluorescence intensity of the probes would be simultaneously changed after the incubation with antioxidants. Thus, the probe can detect antioxidants through both UV–vis and fluorescence spectrophotometers. The dual-modal detection method can achieve rapid detection within 10 min with only 200 μL of samples used. Water soluble total antioxidants in saliva with component proportion fluctuated within a certain range can be well detected. And the obtained results were accurate with high recovery. This dual-modal probe provided an accurate, rapid and noninvasive method to detect water soluble total antioxidants in human body.