Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Bovine Genital Leptospirosis and reproductive disorders of live subfertile cows under field conditions

2021; Elsevier BV; Volume: 261; Linguagem: Inglês

10.1016/j.vetmic.2021.109213

1873-2542

Luíza Aymée, Wilmara Rampinelli Reuter Gregg, Ana Paula Loureiro, Maria Isabel Nogueira Di Azevedo, Juliana Pedrosa, Juliana dos Santos Loria de Melo, Filipe Aníbal Carvalho‐Costa, Guilherme Nunes de Souza, Walter Lilenbaum,

Humic Substances and Bio-Organic Studies

Bovine genital leptospirosis (BGL) is characterized by silent chronic reproductive disorders, most related to early embryonic death leading to estrus repetition, subfertility and abortions. However, most studies were conducted in slaughterhouses, which lacks reproductive and sanitary history of the studied animals. This study aimed to evaluate the occurrence of Leptospira sp. infection in live cows with history of low reproductive efficiency. Blood, urine, cervico-vaginal mucus and uterine fragment were collected from nine cows of the same herd presenting reproductive failure (abortions, estrus repetition and chronic infertility). Serology (MAT) and molecular analysis (PCR and nucleotide sequencing) were performed. Serology showed three (33.3%) seroreactive cows, two to Sejroe and one to Icterohaemorrhagiae serogroups. Six cows (66.7%) presented leptospiral DNA on genital samples, while all urine samples were negative. L. interrogans was identified in five samples, very closely related to strains from Sejroe (n = 3) and Icterohaemorrhagiae (n = 2) serogroups, while L. noguchii was identified in one sample. Results from this preliminary study demonstrates the presence of leptospires on uterus and reinforces the negative impact of leptospiral infection on reproductive tract, highlighting its association with reproductive failures on live animals.