Rapid and Sensitive SERS-Based Lateral Flow Test for SARS-CoV2-Specific IgM/IgG Antibodies
2021; American Chemical Society; Volume: 93; Issue: 36 Linguagem: Inglês
10.1021/acs.analchem.1c02305
ISSN1520-6882
AutoresSupriya Srivastav, Asen Dankov, Mujo Adanalic, Roland Grzeschik, Vi Tran, Sibylle Pagel‐Wieder, Frank Gessler, Ingo Spreitzer, Tatjana Scholz, Barbara S. Schnierle, Olympia E. Anastasiou, Ulf Dittmer, Sebastian Schlücker,
Tópico(s)SARS-CoV-2 and COVID-19 Research
ResumoAs an immune response to COVID-19 infection, patients develop SARS-CoV-2-specific IgM/IgG antibodies. Here, we compare the performance of a conventional lateral flow assay (LFA) with a surface-enhanced Raman scattering (SERS)-based LFA test for the detection of SARS-CoV-2-specific IgM/IgG in sera of COVID-19 patients. Sensitive detection of IgM might enable early serological diagnosis of acute infections. Rapid detection in serum using a custom-built SERS reader is at least an order of magnitude more sensitive than the conventional LFAs with naked-eye detection. For absolute quantification and the determination of the limit of detection (LOD), a set of reference measurements using purified (total) IgM in buffer was performed. In this purified system, the sensitivity of SERS detection is even 7 orders of magnitude higher: the LOD for SERS was ca. 100 fg/mL compared to ca. 1 μg/mL for the naked-eye detection. This outlines the high potential of SERS-based LFAs in point-of-care testing once the interference of serum components with the gold conjugates and the nitrocellulose membrane is minimized.
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