Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Acetylated cashew gum and fucan for incorporation of lycopene rich extract from red guava (Psidium guajava L.) in nanostructured systems: Antioxidant and antitumor capacity

2021; Elsevier BV; Volume: 191; Linguagem: Inglês

10.1016/j.ijbiomac.2021.09.116

1879-0003

Eryka Oliveira de Andrades, João Marcos Antônio Rodrigues da Costa, Francisco Edmar Moreira de Lima Neto, Alyne Rodrigues de Araújo, Fábio de Oliveira Silva Ribeiro, Andreanne Gomes Vasconcelos, Antônia Carla de Jesus Oliveira, José Lamartine Soares‐Sobrinho, Miguel Peixoto de Almeida, Ana P. Carvalho, Jhones do Nascimento Dias, Ingrid Gracielle Martins Silva, Patrícia Albuquerque, Ildinete Silva Pereira, Doralina do Amaral Rabello Ramos, Adriany das Graças Nascimento Amorim, José Roberto de Souza de Almeida Leite, Durcilene Alves da Silva,

Antioxidant Activity and Oxidative Stress

Industrial application of lycopene is limited due to its chemical instability and low bioavailability. This study proposes the development of fucan-coated acetylated cashew gum nanoparticles (NFGa) and acetylated cashew gum nanoparticles (NGa) for incorporation of the lycopene-rich extract from red guava (LEG). Size, polydispersity, zeta potential, nanoparticles concentration, encapsulation efficiency, transmission electron microscopy (TEM) and atomic force microscopy (AFM) were used to characterize nanoparticles. The antioxidant activity was determinated and cell viability was evaluated in the human breast cancer cells (MCF-7) and human keratinocytes (HaCaT) by MTT assay. The toxic effect was evaluated by hemolysis test and by Galleria mellonella model. NFGa showed higher stability than NGa, having a size of 162.10 ± 3.21 nm, polydispersity of 0.348 ± 0.019, zeta potential -30.70 ± 0.53 mV, concentration of 6.4 × 109 nanoparticles/mL and 60% LEG encapsulation. Microscopic analysis revealed a spherical and smooth shape of NFGa. NFGa showed antioxidant capacity by ABTS method and ORAC assay. The NFGa presented significant cytotoxicity against MCF-7 from the lowest concentration tested (6.25-200 μg/mL) and did not affect the cell viability of the HaCaT. NFGa showed non-toxic effect in the in vitro and in vivo models. Therefore, NFGa may have a promising application in LEG stabilization for antioxidant and antitumor purposes.