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Antibiotic sensitivity pattern and risk factor of Escherichia coli isolated from the poultry samples collected in veterinary laboratory, Birendranagar, Surkhet

2021; Medknow; Volume: 5; Issue: 1 Linguagem: Inglês

10.4103/mtsp.mtsp_17_20

2521-0815

Lamichhane Utsav, RS Kushwaha, Hemanta Pokhrel, BK Pandey, K. R. Pandey,

Escherichia coli research studies

A study was conducted from March to April 2020 at Veterinary Laboratory, Birendranagar, to ascertain the antibiotic sensitivity pattern and associated factors of Escherichia coli isolated from the poultry samples. Objective: A total of 50 liver samples were collected and the questionnaire was filled for the corresponding demographic data. The organisms were identified and isolated. Based on the culture characteristics in different agars (nutrient agar, MacConkey agar, and Eosin Methylene Blue (EMB) agar) and the response of the culture toward the biochemical tests, the microorganisms were identified and isolated. Method: Institute of Clinical and Laboratory Standards - recommended Kirby - Bauer disc diffusion method - diffusion method performed for antibiotic susceptibility testing on Muller - Hinton agar. The antibiotic disks used in the study were ciprofloxacin (CP), amikacin (AK), enrofloxacin (EX), gentamicin, azithromycin, tetracycline (TE), and amoxicillin (AMX). Result: The results showed that 36% of the samples were positive for E. coli. Out of 23 birds of age below 28 days, 5 (21.74%) birds were tested positive for E. coli. Similarly, out of 27 birds of age above 28 days, 13 (48.15%) birds were tested positive for E. coli. The relation between E. coli prevalence and the age of the bird was found to be nonsignificant (P > 0.05) according to the Pearson Chi square test. Out of 33 samples from the farm with treated water, 13 (39.39%) of the samples were tested positive for E. coli. Similarly, out of 17 samples from the farm with untreated water, 5 (41.67%) of the samples were tested positive for E. coli. The prevalence of E. coli was significantly (P < 0.05) dependent upon the water treatment in the farm. There were 41 broiler samples out of which 16 (39.02%) samples were tested positive for E. coli. Similarly, there were 9 layer samples, out of which 2 (22.22%) samples were tested positive for the E. coli. There was a nonsignificant (P > 0.05) relation of the prevalence of E. coli with the type of bird. Among the E. coli positive samples(36%), the antibiotic sensitivity pattern was 55.56% for AK, 0.00% for AMX, 61.11% for EX, 38.89% for gentamycin, and 22.22% for TE. Among all the antibiotics, CP showed the highest sensitivity (83.33%) against E. coli. Conclusion: Among all the associated factors, water treatment in the poultry farm showed a significant (P < 0.05) effect on the prevalence of E. coli. Thus, treatment of the water in the farm is recommended to decrease the chances of E. coli prevalence in the farm.