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Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure

2021; Multidisciplinary Digital Publishing Institute; Volume: 11; Issue: 9 Linguagem: Inglês

10.3390/life11090958

2075-1729

Gustavo Gavazzoni Blume, Paulo André Bispo Machado Júnior, Rossana Baggio Simeoni, Giovana Paludo Bertinato, Murilo Sgarbossa Tonial, Seigo Nagashima, Ricardo A. Pinho, Lúcia de Noronha, Márcia Olandoski, Katherine Athayde Teixeira de Carvalho, Júlio César Francisco, Luiz César Guarita-Souza,

Mesenchymal stem cell research

Myocardial infarction (MI) remains the leading cause of cardiovascular death worldwide and a major cause of heart failure. Recent studies have suggested that cell-based therapies with bone marrow stem cells (BMSC) and human amniotic membrane (hAM) would recover the ventricular function after MI; however, the mechanisms underlying these effects are still controversial. Herein, we aimed to compare the effects of BMSC and hAM in a rat model of heart failure. MI was induced through coronary occlusion, and animals with an ejection fraction (EF) < 50% were included and randomized into three groups: control, BMSC, and hAM. The BMSC and hAM groups were implanted on the anterior ventricular wall seven days after MI, and a new echocardiographic analysis was performed on the 30th day, followed by euthanasia. The echocardiographic results after 30 days showed significant improvements on EF and left-ventricular end-sistolic and end-diastolic volumes in both BMSC and hAM groups, without significant benefits in the control group. New blood vessels, desmine-positive cells and connexin-43 expression were also elevated in both BMSC and hAM groups. These results suggest a recovery of global cardiac function with the therapeutic use of both BMSC and hAM, associated with angiogenesis and cardiomyocyte regeneration after 30 days.