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Evaluation of medullary cytokine expression and clinical and laboratory aspects in severe human visceral leishmaniasis

2021; Wiley; Volume: 43; Issue: 12 Linguagem: Inglês

10.1111/pim.12880

1365-3024

Leandro de Freitas Teles, Agostinho Gonçalves Viana, Mariana Santos Cardoso, Guilherme Rafael Gomide Pinheiro, Gabriele Ariadine Bento, Jamille Fernandes Lula, Tânia de Cássia Moreira Soares, Ricardo Toshio Fujiwara, Sílvio Fernando Guimarães de Carvalho,

Parasites and Host Interactions

Abstract Background Visceral leishmaniasis (VL) is a serious public health problem. The factors that can determine whether VL develops and progresses to severe form have not been fully identified, but a specific cellular immune response appears to play a key role. Therefore, understanding immunopathogenesis can be useful in preventing a serious clinical outcome. Materials and Methods Bone marrow samples were collected from patients with severe VL (SVL) or non‐severe VL (NSVL). Cytokine levels and parasitic load were analysed by RT‐qPCR. There is a statistically significant difference in the leukocyte parameter in patients with SVL and NSVL compared with the control patients ( p = .006 and p = .014, respectively). Results Urea, alanine transaminase and albumin parameters had a significant difference p = .036, p = .039 and p = .017, respectively, between SVL and NSVL. Although high levels of IFN‐γ, IL‐10, IL‐6 and TNF‐α were present in all groups of individuals with VL, they were not statistically associated with severity. In patients with active VL, IFN‐γ and IL‐10 were associated, respectively, with a reduction and increase in the parasite load, strong and significant positive association between IFN‐γ and IL‐10 (rho = .627 and p = .003). Conclusion This study demonstrates that VL stimulates an non‐dichotomized inflammatory response between Th1/Th2 and that bone marrow is an important tissue for immune regulation.