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Immunomodulatory and anti-infective effects of Cratylia mollis lectin (Cramoll) in a model of wound infection induced by Staphylococcus aureus

2021; Elsevier BV; Volume: 100; Linguagem: Inglês

10.1016/j.intimp.2021.108094

1878-1705

Mariela Andrea Medeiros Suarez Carneiro, Lucas dos Santos Silva, Roseana Muniz Diniz, Warlison Felipe da Silva Saminez, Patrícia Vieira de Oliveira, Juliana Silva Pereira Mendonça, Arthur Henrique Mendes Colasso, Izadora Souza Soeiro Silva, Jannyson José Braz Jandú, Joicy Cortez de Sá Sousa, Cristiane Santos Silva e Silva Figueiredo, Maria Tereza dos Santos Correia, Luís Cláudio Nascimento da Silva,

Silk-based biomaterials and applications

This work evaluated the immunomodulatory and anti-infective effects of Cratylia mollis lectin (Cramoll) in a model of wound infection induced by S. aureus. Swiss mice were divided into 3 groups (n = 12/group): non-inoculated (Control group); inoculated with S. aureus (Sa group); inoculated with S. aureus and treated with Cramoll (Sa + Cramoll group). In each animal, one lesion (64 mm2) was induced on the back and contaminated with S. aureus (~4.0 × 106 CFU/wound). The treatment with Cramoll (5 μg/animal/day) started 1-day post-infection (dpi) and extended for 10 days. Clinical parameters (wound size, inflammatory aspects, etc.) were daily recorded; while cytokines levels, bacterial load and histological aspects were determined in the cutaneous tissue at 4th dpi or 11th dpi. The mice infected with S. aureus exhibited a delay in wound contraction and the highest inflammatory scores. These effects were impaired by the treatment with Cramoll which reduced the release of key inflammatory mediators (TNF-α, NO, VEGF) and the bacterial load at wound tissue. Histological evaluations showed a restauration of skin structures in the animals treated with Cramoll. Taken together, these results provide more insights about the healing and immunomodulatory properties of Cramoll and suggest this lectin as a lead compound for treatment of wound infection.