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Evaluation of the action of LPSF/GQ-16 on cytokines and PPAR-γ gene expression after in vitro irradiation of peripheral blood mononuclear cells

2021; Taylor & Francis; Volume: 97; Issue: 12 Linguagem: Inglês

10.1080/09553002.2021.1987556

1362-3095

Mariana Brayner Cavalcanti, Lídia Lúcia Bezerra Leite, Diogo Manoel de Queiroz, Thiago de Salazar e Fernandes, Wagner Luís Mendes de Oliveira, Michelly Cristiny Pereira, Maíra Galdino da Rocha Pitta, Moacyr Jesus Barreto de Melo Rêgo, José Araújo dos Santos Júnior, Zahily Herrero Fernández, Diego Rodrigues Cravo Teixeira, Marina Galdino da Rocha Pitta, Ivan da Rocha Pitta, Samuel César Dantas, Robson Cavalcante Veras, Isac Almeida de Medeiros, Edvane Borges da Silva,

Inflammatory mediators and NSAID effects

Patients submitted to radiotherapy (RT) may present in their healthy tissues surrounding the treated tumor, some typical acute inflammatory reactions induced by ionizing radiation (IR). The manifestation of inflammatory processes is a result of exacerbation of the immune system, as a response to radiation exposure, and this can be a limiting factor for RT protocols. To counteract this, some thiazolidinediones, such as LPSF/GQ-16, may be useful for modulating the patient's radioinduced inflammatory response in normal tissues. In this context, the present work aims to evaluate the activity of LPSF/GQ-16 on the levels of cytokines and the expression of the gene PPARγ in mononuclear cells irradiated in vitro, to analyze the immunomodulatory activity of the molecule and its action on radiomitigation.For this, blood samples from eight donors were collected and irradiated with 2 Gy, then the PBMC (peripheral blood mononuclear cells) were cultured and treated with LPSF/GQ-16. The levels of cytokines TNF-α, IFN-γ, IL-2 and IL-4 were quantified by CBA, while the genes of TNF-α, IFN-γ and PPARγ were analyzed by RT-PCR.LPSF/GQ-16 significantly reduced the expression of proinflammatory cytokines (IFN-γ and TNF-α) in irradiated and nonirradiated groups. There was no significant reduction of anti-inflammatory cytokines (IL-2 and IL-4) by LPSF/GQ-16. The mRNA expression of PPAR-γ, IFN-γ and TNF-α in the presence of LPSF/GQ-16 was higher in the nonirradiated sample.LPSF/GQ-16 showed effective activity after irradiation, with an important immunomodulatory activity in irradiated PBMCs.