Produção Nacional
Revisado por pares
Ultrasonic-assisted extraction and automated determination of catalase and lipase activities in bovine and poultry livers using a digital movie-based flow-batch analyzer
2021; Elsevier BV; Volume: 79; Linguagem: Inglês
10.1016/j.ultsonch.2021.105774
ISSN1873-2828
AutoresLucas A. Siqueira, Luciano F. Almeida, Julys Pablo Atayde Fernandes, Mário César Ugulino de Araújo, Ricardo A. C. Lima,
Tópico(s)Meat and Animal Product Quality
ResumoAn ultrasonic reactor (UR) was developed and coupled to a digital movie-based flow-batch analyzer (DM-FBA) for the ultrasonic-assisted extraction (UAE) and fast determination of catalase and lipase activities in bovine and poultry livers. The lab-made UR mainly consisted of a borosilicate glass container and a piezoelectric disc. The DM-FBA mainly consisted of a webcam, an ultrasonic actuator controller, a peristaltic pump, six solenoid valves, a valve driver, a mixing chamber, a magnetic stirrer, an Arduino Mega 2560, and a personal computer. This setup, named UR-DM-FBA, was controlled by custom software. Ultrasound (US) frequency, US power, sonication time, and concentration of extraction agent were optimized using the Taguchi method. Experiments at silent conditions (mechanical stirring at 1500 rpm) were carried out to evaluate extraction efficiency. Optimized parameters for the UAE of catalase were US frequency of 30 kHz, 2.0 mL of Triton X-100, sonication time of 270 s, and US power of 10.8 W. For the UAE of lipase, the optimized parameters were US frequency of 20 kHz, 0.30 mL of triethanolamine, sonication time of 270 s, and US power of 18 W. Catalase and lipase activities obtained with the UR were, on average, 1.9 × 103% and 2.0 × 103% higher than those obtained at silent conditions, respectively, which indicates that that the lab-made UR was capable of extracting these enzymes more efficiently. Determinations using the UR-DM-FBA were highly accurate (relative error ranging from -1.98% to 1.96% for bovine catalase, -0.65% to 0.76% for bovine lipase, -2.03 to 2.08% for poultry catalase, and -0.55% to 0.64% for poultry lipase) and precise (overall coefficient of variation <0.02% for bovine and poultry catalase and 69 h-1) and low sample/reagent consumption (<1.6 mL) were also obtained. Due to the highly efficient UAE, the proposed system can be applied for fast and accurate quantification of lipase and catalase in biological samples with low waste generation.
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