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Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model

2021; Elsevier BV; Volume: 2; Issue: 10 Linguagem: Inglês

10.1016/j.xcrm.2021.100420

2666-3791

Ami Patel, Jewell Walters, Emma L. Reuschel, Katherine Schultheis, Elizabeth M. Parzych, E. Gary, Igor Maricic, Mansi Purwar, Zeena Eblimit, Susanne N. Walker, Diana Guimet, Pratik Bhojnagarwala, Opeyemi S. Adeniji, Arthur Doan, Ziyang Xu, Dustin Elwood, Sophia M. Reeder, Laurent Pessaint, Kevin Y. Kim, Anthony Cook, Neethu Chokkalingam, Brad Finneyfrock, Edgar Tello‐Ruiz, Alan Dodson, Jihae Choi, Alison Generotti, J. P. Harrison, Nicholas J. Tursi, Viviane M. Andrade, Yaya Dia, Faraz I. Zaidi, Hanné Andersen, Mohamed Abdel‐Mohsen, Mark G. Lewis, Kar Muthumani, J. Joseph Kim, Daniel W. Kulp, Laurent Humeau, Stephanie J. Ramos, Trevor R.F. Smith, David B. Weiner, Kate E. Broderick,

Immunotherapy and Immune Responses

Coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus, has had a dramatic global impact on public health and social and economic infrastructures. Here, we assess the immunogenicity and anamnestic protective efficacy in rhesus macaques of an intradermal (i.d.)-delivered SARS-CoV-2 spike DNA vaccine, INO-4800, currently being evaluated in clinical trials. Vaccination with INO-4800 induced T cell responses and induced spike antigen and RBD binding antibodies with ADCP and ADCD activity. Sera from the animals neutralized both the D614 and G614 SARS-CoV-2 pseudotype viruses. Several months after vaccination, animals were challenged with SARS-CoV-2 resulting in rapid recall of anti-SARS-CoV-2 spike protein T cell and neutralizing antibody responses. These responses were associated with lower viral loads in the lung. These studies support the immune impact of INO-4800 for inducing both humoral and cellular arms of the adaptive immune system, which are likely important for providing durable protection against COVID-19 disease.