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First Report of Pestalotiopsis microspora Causing Leaf Blight of Banana in Bangladesh

2021; American Phytopathological Society; Volume: 106; Issue: 5 Linguagem: Inglês

10.1094/pdis-05-21-1120-pdn

1943-7692

Md. Abdullahil Baki Bhuiyan, Shah Mohammad Naimul Islam, Md. Ashraful Islam Bukhari, Md. Abdul Kader, Md. Zahid Hasan Chowdhury, Mohammad Zahangeer Alam, Hasan Mohammad Abdullah, Farhana Jenny,

Plant-Microbe Interactions and Immunity

HomePlant DiseaseVol. 106, No. 5First Report of Pestalotiopsis microspora Causing Leaf Blight of Banana in Bangladesh PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Pestalotiopsis microspora Causing Leaf Blight of Banana in BangladeshMd. Abdullahil Baki Bhuiyan, Shah Mohammad Naimul Islam, Md. Ashraful Islam Bukhari, Md. Abdul Kader, Md. Zahid Hasan Chowdhury, Mohammad Zahangeer Alam, Hasan Mohammad Abdullah, and Farhana JennyMd. Abdullahil Baki Bhuiyan†Corresponding author: Md. A. B. Bhuiyan; E-mail Address: [email protected]https://orcid.org/0000-0003-1872-6566Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh, Shah Mohammad Naimul IslamInstitute of Biotechnology and Genetics Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh, Md. Ashraful Islam BukhariDepartment of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh, Md. Abdul KaderDepartment of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh, Md. Zahid Hasan ChowdhuryInstitute of Biotechnology and Genetics Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh, Mohammad Zahangeer AlamDepartment of Environmental Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh, Hasan Mohammad AbdullahDepartment of Agroforestry and Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh, and Farhana JennySeed Certification Agency, Gazipur, 1701, BangladeshAffiliationsAuthors and Affiliations Md. Abdullahil Baki Bhuiyan1 † Shah Mohammad Naimul Islam2 Md. Ashraful Islam Bukhari1 Md. Abdul Kader1 Md. Zahid Hasan Chowdhury2 Mohammad Zahangeer Alam3 Hasan Mohammad Abdullah4 Farhana Jenny5 1Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh 2Institute of Biotechnology and Genetics Engineering, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh 3Department of Environmental Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh 4Department of Agroforestry and Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh 5Seed Certification Agency, Gazipur, 1701, Bangladesh Published Online:29 Mar 2022https://doi.org/10.1094/PDIS-05-21-1120-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleBanana (Musa sp.) is one of the most important fruit crops in the world. It is also a very popular fruit crop and cultivated widely in Bangladesh. Banana diseases are one of the limiting factors for its production. Recently, a new banana leaf blight was detected in various districts of Gazipur, Bangladesh, with an incidence of 5 to 10% in June 2020 and 15 to 20% in January 2021. The initial symptoms of the disease were narrow, dark-brown lesions (2 to 5 by 5 to 12 mm) that later became irregular brown spots (10 to 15 by 5 to 7 mm). The lesions gradually spread from the middle of the leaf to the margin, and dark-brown spots appeared on the diseased leaves. There was a clear golden-yellowish boundary around the spot. Blighted tissue often covered one-third to one-half of the infected leaves. To isolate the pathogen, 100 diseased leaves were collected from five different locations in Gazipur, Bangladesh. Small pieces (5 by 5 mm) were cut from the margins of lesions and surface sterilized with 75% ethanol for 30 s followed by 1% NaClO for 1 min. The samples were then rinsed three times with sterile distilled water, dried on sterilized filter paper, and placed on 1% water agar at 25°C for 7 days. Hyphal tips were then excised, placed on PDA, and incubated at 25°C for 7 days. From the 20 isolates obtained, the colony characteristics and conidial morphology of three isolates (BLS_BU1, BLS_BU2, and BLS_BU3) were examined. The colony of all of three isolates was white, cottony, and circular in growth. The acervuli were black and covered the entire culture plate. Conidia of all of the isolates were five-celled, fusiform, and wider in the middle than the apical and basal cells. The three internal cells were olivaceous, concolorous, and slightly constricted at the septae; the terminal cells were hyaline (Shi et al. 2015). The conidium measured 20.3 ± 1.2 to 22.7 ± 1.8 by 7.2 ± 0.6 to 8.6 ± 0.4 μm. The mean lengths of the two to four hyaline apical appendages were 20.5 to 30.25 μm and the hyaline basal appendage was 6.1 to 7.6 μm (Han et al. 2019). DNA was extracted from the fungal colony using a DNeasy Plant Mini Kit (Qiagen). PCR was performed with primer pair ITS1/ITS4 ITS region and Sanger sequenced by Macrogen (Seoul, Korea) (White et al. 1990). Isolates BLS_BU1 (MH707065), BLS_BU2 (MH707065), and BLS_BU3 (MH712283) had a sequence similarity of over 99% with GenBank reference Pestalotiopsis microspora (MH707065) of the NCBI database. The reconstructed phylogenetic tree also confirmed their phylogenetic position. To assess pathogenicity, the leaves of four 2-month-old healthy plants were needle wounded and inoculated with a spore suspension (106 spores/ml) harvested from 10-day-old cultures of isolate BLS_BU1. As a control treatment, five leaves of two healthy plants were inoculated with sterile distilled water. Treated plants were maintained in the field with average temperature ranging from 28 to 30°C and relative humidity from 75 to 80%. After 2 weeks, all inoculated leaves produced characteristic symptoms similar to those observed in the field. However, the control plants did not develop any symptoms. P. microspora from the inoculated leaves was successfully reisolated, thus confirming that P. microspora was the cause of leaf blight of banana. To our knowledge, banana appears to be a new host of P. microspora. This is the first report of leaf blight of banana caused by P. microspora in Bangladesh.The author(s) declare no conflict of interest.References:Han, S., et al. 2019. Plant Dis. 103:2963. https://doi.org/10.1094/PDIS-05-19-0937-PDN Link, ISI, Google ScholarShi, H. J., et al. 2015. Plant Dis. 99:1276. https://doi.org/10.1094/PDIS-01-15-0079-PDN Link, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Google ScholarMd. A. B. Bhuiyan and S. M. N. Islam contributed equally to this work.Funding: The authors are grateful to the Ministry of Science and Technology, Government of the People's Republic of Bangladesh for financial support (BS-151-1487) of this research.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 5 May 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 28 Apr 2022Published: 29 Mar 2022First Look: 3 Oct 2021Accepted: 1 Oct 2021 Page: 1518 Information© 2022 The American Phytopathological SocietyFundingMinistry of Science and Technology, Government of the People's Republic of BangladeshGrant/Award Number: BS-151-1487Keywordsleaf blightlesionnecrosisPestalotiopsisThe author(s) declare no conflict of interest.PDF downloadCited byBananaSqueezeNet: A Very Fast, Lightweight Convolutional Neural Network for Diagnosis of Three Prominent Banana Leaf DiseasesSmart Agricultural Technology