Comparative Proteomics Reveals the Effect of the Transcriptional Regulator Sp13016 on Butenyl-Spinosyn Biosynthesis in Saccharopolysp ora pogona
2021; American Chemical Society; Volume: 69; Issue: 42 Linguagem: Inglês
10.1021/acs.jafc.1c03654
ISSN1520-5118
AutoresJianli Tang, Haocheng He, Yunlong Li, Zhudong Liu, Ziyuan Xia, Li Cao, Zirong Zhu, Ling Shuai, Yang Liu, Qianqian Wan, Yuewen Luo, Youming Zhang, Jie Rang, Liqiu Xia,
Tópico(s)Fungal Biology and Applications
ResumoButenyl-spinosyn is a highly effective and broad-spectrum biopesticide produced by Saccharopolyspora pogona. However, the yield of this compound is difficult to increase because the regulatory mechanism of secondary metabolism is still unknown. Here, the transcriptional regulator Sp13016 was discovered to be highly associated with butenyl-spinosyn synthesis and bacterial growth. Overexpression of sp13016 improved butenyl-spinosyn production to a level that was 2.84-fold that of the original strain, while deletion of sp13016 resulted in a significant decrease in yield and growth inhibition. Comparative proteomics revealed that these phenotypic changes were attributed to the influence of Sp13016 on the central carbon metabolism pathway to regulate the supply of precursors. Our research helps to reveal the regulatory mechanism of butenyl-spinosyn biosynthesis and provides a reference for increasing the yield of natural products of Actinomycetes.
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