Portal de Periódicos da CAPES

Tropane alkaloid production and riboflavin excretion by Hyoscyamus niger L. hairy root cultures

2004; Estonian Academy Publishers; Volume: 53; Issue: 1 Linguagem: Inglês

10.3176/biol.ecol.2004.1.02

1736-7611

Elmar Arak, K. Pudersell, Ain Raal, R. Vardja, T. Vardja,

Plant tissue culture and regeneration

The production of tropane alkaloids and excretion of riboflavin was comparatively investigated in hairy root clones of Hyoscyamus niger transformed with Agrobacterium rhizogenes strain LBA 9402 and wild-type (WT) root culture of H. niger.From six selected hairy root clones, one clone (C-1) was relatively similar to WT roots in morphological features and growth intensity.The other, fast-growing (FG) root clones grew twice better than WT roots and had very variable morphological features.Clone C-1 produced, depending on the medium (MS, B5, Knop-M), 5 to 15 times more scopolamine and 1.5 to 2 times more hyoscyamine than WT roots.Most of the FG root clones produced less tropane alkaloids than WT roots, but there was a strong dependence on the composition of media.The excretion of riboflavin from C-1 clone was somewhat lower than that from the WT roots.The excretion of riboflavin from the FG root clones occurred at a detection level, but increased under iron deficiency of media.