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Analysis of the complete genome of HBV genotypes F and H found in Brazil and Mexico using the next generation sequencing method

2021; Elsevier BV; Volume: 27; Linguagem: Inglês

10.1016/j.aohep.2021.100569

2659-5982

Patrícia Oliveira Gionda, Michele Soares Gomes‐Gouvêa, Fernanda de Mello Malta, Pedro Henrique Sebe Rodrigues, Ana Paula Moreira Salles, Rodrigo dos Santos Francisco, Alexis José-Ábrego, Sonia Román, Arturo Panduro, João Renato Rebello Pinho,

Vibrio bacteria research studies

Hepatitis B Virus is classified into ten different genotypes (A- J). Genotypes F and H cluster apart from others in phylogenetic trees and is particularly frequent in the Americas. The aim of this study was to sequence complete genomes of samples of HBV genotypes F and H from Brazil and Mexico using next generation sequencing (NGS) and to study relevant characteristics for the disease associated with this virus.Ninety plasma samples with detectable HBV DNA belonging to the F (n=59) and H (n=31) genotypes were submitted to amplification of the complete HBV genome by three different methologies. Data analysis was developed using bioinformatics tools for quality assurance and comprehensive coverage of the genome. Sequences were aligned with reference sequences for subgenotyping and detecting variants in relevant positions. A phylogenetical tree was constructed using Bayesian methods.HBV genome of 31 samples were amplified and 18 of them were sequenced (HBV/F=16 and HBV/H=2). One genotype F sample was co-infected with the F1b and F3 subgenotypes, while the other samples were all F2a subgenotype. Two genotype H samples clustered with other Mexican sequences. The main variants observed were found in preS and S genes (7/18) and mutations in the precore/core region (11/18).A NGS methodology was applied to F and H genotypes samples from Mexico and Brazil to fully characterize their sequences. This methodology will be relevant for clinical and epidemiological studies of hepatitis B in Latin America.