Isolation and identification of an arabinogalactan extracted from pistachio external hull: Assessment of immunostimulatory activity
2021; Elsevier BV; Volume: 373; Linguagem: Inglês
10.1016/j.foodchem.2021.131416
ISSN1873-7072
AutoresMariem Hamed, Elisabete Coelho, Rita Bastos, Dmitry V. Evtuguin, Sónia S. Ferreira, Tânia Lima, Manuel Vilanova, Assaâd Sila, Manuel A. Coimbra, Ali Bougatef,
Tópico(s)Phytochemicals and Antioxidant Activities
ResumoThis work studies the extraction and purification of a novel arabinogalactan from pistachio external hull. It was extracted with a simple method from pistachio hull which is considered as unexploited waste. Based on the results of sugar analysis by GC-FID, glycosidic linkage by GC-MS, NMR spectroscopy, and molecular weight by Size Exclusion Chromatography, pistachio hull water soluble polysaccharides (PHWSP) were identified as a type II arabinogalactan (AG), with characteristic terminally linked α-Araf, (α1 → 5)-Araf, (α1 → 3,5)-Araf, terminally linked β-Galp, (β1 → 6)-Galp, and (β1 → 3,6)-Galp. DEPT-135, HSQC, HMBC and COSY NMR data suggested the presence of (β1 → 3)-Galp mainly branched at O-6 with (β1 → 6)-Galp chains, α-Araf chains, and terminally linked α-Araf. These AG from pistachio external hulls showed in vitro stimulatory activity for B cells, suggesting their possible use as an immunological stimulant in nutraceutical and biomedical applications.
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