Surface Plasmon-Coupled Dual Emission Platform for Ultrafast Oxygen Monitoring after SARS-CoV-2 Infection
2021; American Chemical Society; Volume: 6; Issue: 12 Linguagem: Inglês
10.1021/acssensors.1c01665
ISSN2379-3694
AutoresBebeto Rai, Robert Malmberg, Venkatesh Srinivasan, Kalathur Mohan Ganesh, Naga Sai Visweswar Kambhampati, Abhay Andar, Govind Rao, Carani B. Sanjeevi, Koushik Venkatesan, Sai Sathish Ramamurthy,
Tópico(s)Biosensors and Analytical Detection
ResumoThe outbreak of the COVID-19 pandemic has had a major impact on the health and well-being of people with its long-term effect on lung function and oxygen uptake. In this work, we present a unique approach to augment the phosphorescence signal from phosphorescent gold(III) complexes based on a surface plasmon-coupled emission platform and use it for designing a ratiometric sensor with high sensitivity and ultrafast response time for monitoring oxygen uptake in SARS-CoV-2-recovered patients. Two monocyclometalated Au(III) complexes, one having exclusively phosphorescence emission (λPL = 578 nm) and the other having dual emission, fluorescence (λPL = 417 nm) and phosphorescence (λPL = 579 nm), were studied using the surface plasmon-coupled dual emission (SPCDE) platform for the first time, which showed 27-fold and 17-fold enhancements, respectively. The latter complex having the dual emission was then used for the fabrication of a ratiometric sensor for studying the oxygen quenching of phosphorescence emission with the fluorescence emission acting as an internal standard. Low-cost poly (methyl methacrylate) (PMMA) and biodegradable wood were used to fabricate the microfluidic chips for oxygen monitoring. The sensor showed a high sensitivity with a limit of detection ∼ 0.1%. Furthermore, real-time oxygen sensing was carried out and the response time of the sensor was calculated to be ∼0.2 s. The sensor chip was used for monitoring the oxygen uptake in SARS-CoV-2-recovered study participants, to assess their lung function post the viral infection.
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