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Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors

2021; National Academy of Sciences; Volume: 118; Issue: 44 Linguagem: Inglês

10.1073/pnas.2111031118

1091-6490

Kira Gysel, Mette Laursen, Mikkel B. Thygesen, Damiano Lironi, Zoltán Bozsóki, Christian T. Hjuler, Nicolai Maolanon, Jeryl Cheng, P. Björk, Maria Vinther, Lene Madsen, Henriette Rübsam, Artur Muszyński, Arshia Ghodrati, Parastoo Azadi, John T. Sullivan, Clive W. Ronson, Knud J. Jensen, Mickaël Blaise, Simona Radutoiu, Jens Stougaard, Kasper R. Andersen,

Plant-Microbe Interactions and Immunity

Significance Plant cell surface receptors perceive carbohydrate signaling molecules and hereby establish communication with surrounding microbes. Genetic studies have identified two different classes of lysin motif receptor kinases as gatekeepers that together trigger the symbiotic pathway in plants; however, no structural or functional data of the perception mechanisms switching these receptors from resting state into activation is known. In this study, we use structural biology, biochemical, and genetic approaches to demonstrate how the NFP/NFR5 class of lipochitooligosaccharide (LCO) receptors discriminate bacterial symbionts based on a kinetic proofreading mechanism that controls receptor activation and signaling specificity. We show that the LCO binding site can be engineered to support symbiotic functions, which greatly advance future opportunities for receptor engineering in legumes and nonlegumes.