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Artigo Produção Nacional Revisado por pares
BIBTEX RIS

Lignin from Morinda citrifolia leaves: Physical and chemical characterization, in vitro evaluation of antioxidant, cytotoxic, antiparasitic and ultrastructural activities

2021; Elsevier BV; Volume: 193; Linguagem: Inglês

10.1016/j.ijbiomac.2021.11.013

ISSN

1879-0003

Autores

Paula Roberta da Silva, Maria do Carmo Alves de Lima, Thammyris Pires de Souza, Jana Messias Sandes, Alice da Conceição Alves de Lima, Pedró José Rolim Neto, Fábio André Brayner dos Santos, Luiz Carlos Alves, Rosali Maria Ferreira da Silva, George Jackson de Moraes Rocha, Iranildo José da Cruz Filho,

Tópico(s)

Dyeing and Modifying Textile Fibers

Resumo

In this work, we investigated in vitro the antioxidant, cytotoxic and anti-leishmanial activities of a lignin extracted from the leaves of Morinda citrifolia. Initially, an analysis of the composition of the sheets was performed, then the lignin was obtained by alkaline delignification and characterized by different techniques: elemental analysis, FT-R, UV-vis, HSQC-NMR, thermal analysis, Py-GC/MS and by GPC. The results showed that the leaves had in their composition cellulose (31.29%), hemicellulose (25.01%), lignin (18.34%), extractives (14.39%) and ash (10.03%). The lignin extraction yield was 89.8%. The lignin obtained is of the GSH type with the following contents 79.39%, 13.58% and 7.03% respectively. Furthermore, it is low molecular weight and thermally stable. It had a phenolic content of 93.3 mg GAE/g and low antioxidant activity. In macrophage cytotoxicity assays, it presented a CC50 of 31.0 μg/mL, showing less toxicity than amphotericin B. In assays against the promastigote forms of Leishmania amazonensis, lignin presented an IC50 of 29.56 μg/mL, a less effective concentration than amphotericin B (IC50 = 0.14 μg/mL). However, it was able to promote inhibition of the parasites, a fact confirmed by structural changes. These findings reinforce that M. citrifolia lignin is a promising macromolecule for use as an antiparasitic and antioxidant agent.

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