Produção Nacional
Revisado por pares
YAP signaling in preovulatory granulosa cells is critical for the functioning of the EGF network during ovulation
2021; Elsevier BV; Volume: 541; Linguagem: Inglês
10.1016/j.mce.2021.111524
ISSN1872-8057
AutoresEsdras Corrêa Dos Santos, Ariane Lalonde-Larue, Alfredo Quites Antoniazzi, Marcos Henrique Barreta, Christopher A. Price, Paulo Bayard Dias Gonçalves, Valério Marques Portela, Gustavo Zamberlam,
Tópico(s)Reproductive Biology and Fertility
ResumoFailure to ovulate is a major cause of infertility. The critical pathway that induces ovulation involves the EGF and MAPK phosphorylation, but studies in rodents have suggested that the Hippo activator, YAP, is also involved. It is unknown whether YAP-dependent transcriptional activity is important for the LH- or EGF-induced ovulatory cascade in monovulatory species such as the cow. Using a well-defined preovulatory GC culture system, we employed pharmacological inhibitors to demonstrate that YAP signaling is critical for expression of EGFR and downstream target genes EREG, EGR1 and TNFAIP6. Most importantly, by using an ultrasound guided follicle injection system, we also showed that the classic Hippo signaling inhibitor Verteporfin inhibits GnRH-induced ovulation in vivo in cattle. In conclusion, YAP transcriptional activity is critical for EGF-like cascade induced by LH to promote ovulation in a monovulatory species.
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