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Purified phytochrome influences in vitro transcription in rye nuclei

1984; Springer Nature; Volume: 3; Issue: 13 Linguagem: Inglês

10.1002/j.1460-2075.1984.tb02261.x

1460-2075

Dieter Ernst, Dieter Oesterhelt,

Plant Genetic and Mutation Studies

Article20 December 1984free access Purified phytochrome influences in vitro transcription in rye nuclei Dietrich Ernst Dietrich Ernst Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG Search for more papers by this author Dieter Oesterhelt Dieter Oesterhelt Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG Search for more papers by this author Dietrich Ernst Dietrich Ernst Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG Search for more papers by this author Dieter Oesterhelt Dieter Oesterhelt Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG Search for more papers by this author Author Information Dietrich Ernst1 and Dieter Oesterhelt1 1Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG The EMBO Journal (1984)3:3075-3078https://doi.org/10.1002/j.1460-2075.1984.tb02261.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Nuclei isolated from dark-grown seedlings of rye by Percoll density gradient centrifugation incorporate radioactive UTP into RNA. Transcription is reduced to ˜50% by the addition of α-amanitin (5 μg/ml) and correspondingly a decrease of the label in the mRNA fraction is seen on agarose gels. Purified 124-kd phytochrome in its far-red absorbing form (Pfr) increases the incorporation of labeled UTP by 40-70% above untreated controls, whereas pre-illumination with far-red light or addition of α-amanitin prevents this effect. Nuclei isolated from light-grown seedlings show only an increase of ˜5% upon addition of Pfr. Other proteins such as bovine serum albumin or cytochrome c do not enhance the rate of transcription. We conclude that the accumulation of mRNA species is influenced by phytochrome in its Pfr form. Previous ArticleNext Article Volume 3Issue 131 December 1984In this issue RelatedDetailsLoading ...