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Genetics of Collybia velutipes, I. Mating type and barrage phenomenon (1).*

1954; Botanical Society of Japan; Volume: 67; Issue: 787-788 Linguagem: Inglês

10.15281/jplantres1887.67.23

2185-3835

Tsuneo TAKEMARU,

Viral Infectious Diseases and Gene Expression in Insects

Winter fungus, Collybia velutipes (Curt.)Fr., is a common edible agaric, whose fruit-bodies are frequently found growing on the trunks or the stumps of various broad-leaved trees during winter.In this fungus, which is readily grown in culture, it is possible to control the conditions of culture in such a way that the fruit-bodies can be aseptically produced in glass container when desired.In the present paper, the mating type of C. velutipes and its relation with barrage phenomenon observed between monosporous cultures of the fungus will be reported. Material and MethodsFruit-bodies ~f C. vetutipes were found in December, 1950 developing on the slants of potato extract agar in test tubes into which a piece of the dikaryotic mycelia had been aseptically inoculated a month ago.From one of these fruitbodies, the spore deposit was obtained which provided a series of pure monosporous cultures used in the present study.The isolation of the single basidiospore was followed by the ordinary plate method.Two weeks after twenty-one monosporous mycelia of the fungus had been isolated, they were mated in all possible combinations.The matings .were made by inoculating each piece of any two monosporous mycelia side by side on the identical slants of nutrient agar in test tubes.Agar plates in Petri dishes were also used when they were more convenient for observing barrage effect.Potato extract agar and malt extract agar were most commonly used as the nutrient agar.The writer's modification of Fries III synthetic agar (denoted shortly as MFIII), which contains no biotin, is also a very satisfactory medium for the growth of mycelia, and is made according to the following formula : sucrose, 30.0 g.; ammonia tartrate, 5.0 g.; NH4NO3, 1.0 g.; KH2PO4, 1.0 g.; MgSO4 7H2O, 0.5 g.; CaC12, 0.1 g.; NaCI, 0.1 g.; agar, 20.0 g.; distilled water, 1 liter.The vegetative mycelia were incubated at 25°C.Conditions for the formation of fruit-bodies :---When a dikaryotic mycelium covering the entire surface of nutrient agar in a glass container is in suitable environ-