Artigo Acesso aberto

Genotyping of Single Nucleotide Polymorphisms by 5′ Nuclease Allelic Discrimination

2012; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-61779-842-9_10

ISSN

1940-6029

Autores

Mari Malkki, Effie W. Petersdorf,

Tópico(s)

CRISPR and Genetic Engineering

Resumo

Real-time quantitative PCR is an efficient method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). In this chapter, we describe the 5′ nuclease allelic discrimination assay for genotyping biallelic SNPs.

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