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Molecular analysis of the yellow locus of Drosophila

1986; Springer Nature; Volume: 5; Issue: 13 Linguagem: Inglês

10.1002/j.1460-2075.1986.tb04688.x

1460-2075

William Chia, G. Howes, Mark O. Martin, Yuan B. Meng, Kevin Moses, Stuart I. Tsubota,

Plant Molecular Biology Research

Article20 December 1986free access Molecular analysis of the yellow locus of Drosophila William Chia William Chia Department of Biochemistry and Unit of Molecular Genetics, University of Bristol, Bristol BS8 1TD, UK Search for more papers by this author Geoff Howes Geoff Howes Department of Biochemistry and Unit of Molecular Genetics, University of Bristol, Bristol BS8 1TD, UK Search for more papers by this author Mark Martin Mark Martin Department of Biochemistry and Unit of Molecular Genetics, University of Bristol, Bristol BS8 1TD, UK Search for more papers by this author Yuan B. Meng Yuan B. Meng Department of Biochemistry and Unit of Molecular Genetics, University of Bristol, Bristol BS8 1TD, UK Search for more papers by this author Kevin Moses Kevin Moses Department of Biochemistry, University of California, Berkeley, CA 94270, USA Search for more papers by this author Stuart Tsubota Stuart Tsubota Department of Biology, Princeton University, Princeton, NJ 08544, USA Search for more papers by this author William Chia William Chia Department of Biochemistry and Unit of Molecular Genetics, University of Bristol, Bristol BS8 1TD, UK Search for more papers by this author Geoff Howes Geoff Howes Department of Biochemistry and Unit of Molecular Genetics, University of Bristol, Bristol BS8 1TD, UK Search for more papers by this author Mark Martin Mark Martin Department of Biochemistry and Unit of Molecular Genetics, University of Bristol, Bristol BS8 1TD, UK Search for more papers by this author Yuan B. Meng Yuan B. Meng Department of Biochemistry and Unit of Molecular Genetics, University of Bristol, Bristol BS8 1TD, UK Search for more papers by this author Kevin Moses Kevin Moses Department of Biochemistry, University of California, Berkeley, CA 94270, USA Search for more papers by this author Stuart Tsubota Stuart Tsubota Department of Biology, Princeton University, Princeton, NJ 08544, USA Search for more papers by this author Author Information William Chia1, Geoff Howes1, Mark Martin1, Yuan B. Meng1, Kevin Moses2 and Stuart Tsubota3 1Department of Biochemistry and Unit of Molecular Genetics, University of Bristol, Bristol BS8 1TD, UK 2Department of Biochemistry, University of California, Berkeley, CA 94270, USA 3Department of Biology, Princeton University, Princeton, NJ 08544, USA The EMBO Journal (1986)5:3597-3605https://doi.org/10.1002/j.1460-2075.1986.tb04688.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The yellow (y) locus of Drosophila controls the pattern of pigmentation of the adult cuticle and larval mouth parts. Mutations of y belong to two phenotypic classes; type1 mutants exhibit a total loss of pigmentation from all parts of the cuticle and type 2 mutants show allele-specific mosaic pigmentation patterns. We have cloned y by P-element tagging followed by chromosome walking. We demonstrate that a 2.1-kb transcript which spans 4.6 kb of genomic sequence is the y transcript. The location of this transcript and the putative protein it encodes has been defined by nuclease protection, primer extension and DNA sequencing experiments. A 12-kb genomic fragment containing this transcript along with 2.8 kb of 5′-flanking sequence and 4.6 kb of 3′-flanking sequence is sufficient for the wild-type expression of the larval and adult cuticle phenotypes in germ-line transformants. The majority (12/14) of type1 mutants show structural lesions within exon coding portions of y. Most (6/7) of the type2 structural lesions map 5′ to the start of y transcription. One type2 mutant which contains an insertion within the 5′-transcribed but non-translated region appears to have resulted from the internal deletion of a P-element associated with a type1 allele. Previous ArticleNext Article Volume 5Issue 131 December 1986In this issue RelatedDetailsLoading ...