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BIBTEX RIS

Alternative solution for ex vivo lung perfusion, experimental study on donated human lungs non-accepted for transplantation

2015; Sociedade Brasileira Para o Desenvolvimento da Pesquisa em Cirurgia; Volume: 30; Issue: 5 Linguagem: Inglês

10.1590/s0102-865020150050000008

ISSN

1678-2674

Autores

Lucas Matos Fernandes, Alessandro Wasum Mariani, Israel Lopes de Medeiros, Marcos Naoyuki Samano, Luís Gustavo Abdalla, Aristides Tadeu Correia, Natália Aparecida Nepomuceno, Mauro Canzian, Paulo Manuel Pêgo‐Fernandes,

Tópico(s)

Organ Donation and Transplantation

Resumo

PURPOSE: To evaluate a new perfusate solution to be used for ex vivo lung perfusion. METHODS: Randomized experimental study using lungs from rejected brain-dead donors harvested and submitted to 1 hour of ex vivo lung perfusion (EVLP) using mainstream solution or the alternative. RESULTS: From 16 lungs blocs tested, we found no difference on weight after EVLP: Steen group (SG) = 1,097±526g; Alternative Perfusion Solution (APS) = 743±248g, p=0.163. Edema formation, assessed by Wet/dry weigh ratio, was statistically higher on the Alternative Perfusion Solution group (APS = 3.63 ± 1.26; SG = 2.06 ± 0.28; p = 0.009). No difference on PaO2 after EVLP (SG = 498±37.53mmHg; APS = 521±55.43mmHg, p=0.348, nor on histological analyses: pulmonary injury score: SG = 4.38±1.51; APS = 4.50±1.77, p=0.881; apoptotic cells count after perfusion: SG = 2.4 ± 2.0 cells/mm2; APS = 4.8 ± 6.9 cells/mm2; p = 0.361). CONCLUSION: The ex vivo lung perfusion using the alternative perfusion solution showed no functional or histological differences, except for a higher edema formation, from the EVLP using Steen Solution(r) on lungs from rejected brain-dead donors.

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