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Teratogenic Effects of Phenytoin on the Length of Bones of the Uper and Lower Extremities Development on Mice Fetuses (NMRI)

2013; Volume: 3; Issue: 6 Linguagem: Inglês

2165-9036

Bibi Mahboobeh Torabi, Sepideh Shadab, Shams Abad, Abdolhossein Shiravi,

Pharmaceutical studies and practices

Teratogenic effects of phenytoin on ossification of the bones of the upper and lower extremities on mice fetuses (NM RI). This study was desigened to determine the teratogenic effects of phenytoin on ossificat ion of the upper and lower extremities on mice fetuses (NM RI). In this study used 40 white s mall pregnant mice (NMRI type) were divided in to three experimental groups (I,II,III) and one control group. Three experimental groups received 60,75 and 90 ml/ kg/day of phenytoin solution in normal salin 0.2 cc volu m fro m the day 6.5 (GD 6.5) to day 14.5 (GD 14.5) of pregnancy intraperitoneally (ip) and the control (group received normal saline with the same volu me the mice on the eighteenth and a half day of p regnancy were sacrificial and the fetuses staind with Alizarin Red S/ A lcian blue and length of bones of the upper and lower extremit ies were measured with stereomicroscope that was equipped with a calibrate lens. Data were analysed by ANOVA T-test and tukey using SPSS software. Results showed that average of bones length of upper and lower extremit ies in three experimental groups were significauntly shorter co mpared with the control group (p<0.005). Also this study revealed that phenytoin can reduce ossifsication of the upper and lower organs if this drug is used subsequent to organogenesis stage .