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DF 31, a sperm decondensation factor from Drosophila melanogaster: purification and characterization.

1995; Springer Nature; Volume: 14; Issue: 8 Linguagem: Inglês

10.1002/j.1460-2075.1995.tb07160.x

1460-2075

Gilles Crevel, Sue Cotterill,

Animal Genetics and Reproduction

Research Article18 April 1995free access DF 31, a sperm decondensation factor from Drosophila melanogaster: purification and characterization. G. Crevel G. Crevel Marie Curie Research Institute, Oxted, Surrey, UK. Search for more papers by this author S. Cotterill S. Cotterill Marie Curie Research Institute, Oxted, Surrey, UK. Search for more papers by this author G. Crevel G. Crevel Marie Curie Research Institute, Oxted, Surrey, UK. Search for more papers by this author S. Cotterill S. Cotterill Marie Curie Research Institute, Oxted, Surrey, UK. Search for more papers by this author Author Information G. Crevel1 and S. Cotterill1 1Marie Curie Research Institute, Oxted, Surrey, UK. The EMBO Journal (1995)14:1711-1717https://doi.org/10.1002/j.1460-2075.1995.tb07160.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We have purified to homogeneity a Drosophila protein which is able to decondense Xenopus sperm chromatin. This protein, which we have called DF 31, is a heat-stable phosphoprotein which displays a molecular weight of 31 kDa on SDS-PAGE, but which has an apparent molecular weight of > 200 kDa on gel filtration. We show that DF 31 decondenses sperm DNA by displacement of sperm-specific proteins. In addition to its sperm decondensation activity, DF 31 is also able to facilitate nucleosome loading on both decondensed sperm DNA and on naked DNA template. The reaction as catalysed by DF 31 is quite efficient; however, the nucleosomes appear to be loaded randomly onto the DNA, not in regular arrays. Although the mechanism by which DF 31 aids nucleosome loading is not yet clear, it most probably occurs through binding of DF 31 to core histones. Previous ArticleNext Article Volume 14Issue 81 April 1995In this issue RelatedDetailsLoading ...