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The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton.

1994; Springer Nature; Volume: 13; Issue: 4 Linguagem: Inglês

10.1002/j.1460-2075.1994.tb06318.x

1460-2075

Susanne Pistor, Trinad Chakraborty, Kirsten Niebuhr, Eugen Domann, J. Wehland,

Listeria monocytogenes in Food Safety

Research Article15 February 1994free access The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton. S. Pistor S. Pistor Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. Search for more papers by this author T. Chakraborty T. Chakraborty Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. Search for more papers by this author K. Niebuhr K. Niebuhr Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. Search for more papers by this author E. Domann E. Domann Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. Search for more papers by this author J. Wehland J. Wehland Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. Search for more papers by this author S. Pistor S. Pistor Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. Search for more papers by this author T. Chakraborty T. Chakraborty Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. Search for more papers by this author K. Niebuhr K. Niebuhr Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. Search for more papers by this author E. Domann E. Domann Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. Search for more papers by this author J. Wehland J. Wehland Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. Search for more papers by this author Author Information S. Pistor1, T. Chakraborty1, K. Niebuhr1, E. Domann1 and J. Wehland1 1Gesellschaft für Biotechnologische Forschung, Abteilung Mikrobiologie, Germany. The EMBO Journal (1994)13:758-763https://doi.org/10.1002/j.1460-2075.1994.tb06318.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Listeria monocytogenes, a facultative intracellular pathogen, employs actin and other microfilament-associated proteins to move through the host cell cytoplasm. Isogenic mutants of L. monocytogenes lacking the surface-bound ActA polypeptide no longer interact with cytoskeletal elements and are, as a consequence, non-motile (Domann et al., 1992, EMBO J., 11, 1981-1990; Kocks et al., 1992, Cell, 68, 521-531). To investigate the interaction of ActA with the microfilament system in the absence of other bacterial factors, the listerial actA gene was expressed in eukaryotic cells. Immunofluorescence studies revealed that the complete ActA, including its C-terminally located bacterial membrane anchor, colocalized with mitochondria in transfected cells. When targeted to mitochondria, the ActA polypeptide recruited actin and alpha-actinin to these cellular organelles with concomitant reorganization of the microfilament system. Removal of the internal proline-rich repeat region of ActA completely abrogated interaction with cytoskeletal components. Our results identify the ActA polypeptide as a nucleator of the actin cytoskeleton and provide the first insights into the molecular nature of such controlling elements in microfilament organization. Previous ArticleNext Article Volume 13Issue 41 February 1994In this issue RelatedDetailsLoading ...