Studying Protein–Protein Interactions via Blot Overlay or Far Western Blot
2004; Humana Press; Linguagem: Inglês
10.1385/1-59259-762-9
Autores Tópico(s)Enzyme Structure and Function
ResumoBlot overlay is a useful method for studying protein-protein interactions. This technique involves fractionating proteins on SDS-PAGE, blotting to nitrocellulose or PVDF membrane, and then incubating with a probe of interest. The probe is typically a protein that is radiola-beled, biotinylated, or simply visualized with a specific antibody. When the probe is visualized via antibody detection, this technique is often referred to as "Far Western blot." Many different kinds of protein-protein interactions can be studied via blot overlay, and the method is applicable to screens for unknown protein-protein interactions as well as to the detailed characterization of known interactions.
Referência(s)