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Structural Genomics of Pathogenic Protozoa: an Overview

2008; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-60327-058-8_33

1940-6029

Erkang Fan, David Baker, Stanley Fields, Michael H. Gelb, Frederick S. Buckner, Wesley C. Van Voorhis, Eric M. Phizicky, Mark E. Dumont, Christopher Mehlin, Elizabeth J. Grayhack, Mark A. Sullivan, Christophe L. M. J. Verlinde, George T. DeTitta, Deirdre R. Meldrum, E.A. Merritt, Thomas Earnest, M. Soltis, Frank Zucker, Peter J. Myler, Lori W. Schoenfeld, David E. Kim, Liz Worthey, Doug LaCount, Marissa Vignali, Jizhen Li, Somnath Mondal, Archna P. Massey, Brian J. Carroll, Stacey Gulde, Joseph R. Luft, Larry A. DeSoto, Mark R. Holl, J.M. Caruthers, Jürgen Bosch, M.A. Robien, T.L. Arakaki, Margaret A. Holmes, Isolde Le Trong, Wim G. J. Hol,

Toxoplasma gondii Research Studies

The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of “co-crystallants,” and capillary crystallization. “Fragment cocktail crystallography” for medical structural genomics is also described.