First Report of Fusarium oxysporum f. sp. cubense Tropical Race 4 Causing Fusarium Wilt in Cavendish Bananas in Peru
2021; American Phytopathological Society; Volume: 106; Issue: 8 Linguagem: Inglês
10.1094/pdis-09-21-1951-pdn
ISSN1943-7692
AutoresR. Acuña, Mathieu Rouard, Ana M. Leiva, Caroline Marques, J. A. Olortegui, Cledy Ureta Sierra, Rosa María Cabrera Pintado, Juan Carlos Rojas, Diana López, Alberto Cenci, Wilmer J. Cuéllar, Miguel Dita,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 106, No. 8First Report of Fusarium oxysporum f. sp. cubense Tropical Race 4 Causing Fusarium Wilt in Cavendish Bananas in Peru PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Fusarium oxysporum f. sp. cubense Tropical Race 4 Causing Fusarium Wilt in Cavendish Bananas in PeruR. Acuña, M. Rouard, A. M. Leiva, C. Marques, J. A. Olortegui, C. Ureta, R. M. Cabrera-Pintado, J. C. Rojas, D. Lopez-Alvarez, A. Cenci, W. J. Cuellar, and M. DitaR. AcuñaServicio Nacional de Sanidad Agraria, Servicio Nacional de Sanidad Agraria del Peru, La Molina, Lima 12, Perú, M. Rouardhttps://orcid.org/0000-0003-0284-1885Bioversity International, Parc Scientifique Agropolis II, Montpellier, 34397, France, A. M. LeivaAlliance of Bioversity International and CIAT, Cali, 763537, Colombia, C. MarquesServicio Nacional de Sanidad Agraria, Servicio Nacional de Sanidad Agraria del Peru, La Molina, Lima 12, Perú, J. A. OlorteguiServicio Nacional de Sanidad Agraria, Servicio Nacional de Sanidad Agraria del Peru, La Molina, Lima 12, Perú, C. Uretahttps://orcid.org/0000-0001-5563-261XServicio Nacional de Sanidad Agraria, Servicio Nacional de Sanidad Agraria del Peru, La Molina, Lima 12, Perú, R. M. Cabrera-Pintadohttps://orcid.org/0000-0003-2396-8674Instituto Nacional de Innovación Agraria, Lima, 2791, Peru, J. C. Rojashttps://orcid.org/0000-0001-7538-2275Instituto Nacional de Innovación Agraria, Lima, 2791, Peru, D. Lopez-AlvarezInstituto Nacional de Innovación Agraria, Lima, 2791, Peru, A. CenciBioversity International, Parc Scientifique Agropolis II, Montpellier, 34397, France, W. J. Cuellarhttps://orcid.org/0000-0003-4702-3237Alliance of Bioversity International and CIAT, Cali, 763537, Colombia, and M. Dita†Corresponding author: M. Dita; E-mail Address: [email protected]https://orcid.org/0000-0002-0496-4267Alliance of Bioversity International and CIAT, Cali, 763537, ColombiaAffiliationsAuthors and Affiliations R. Acuña1 M. Rouard2 A. M. Leiva3 C. Marques1 J. A. Olortegui1 C. Ureta1 R. M. Cabrera-Pintado4 J. C. Rojas4 D. Lopez-Alvarez4 A. Cenci2 W. J. Cuellar3 M. Dita3 † 1Servicio Nacional de Sanidad Agraria, Servicio Nacional de Sanidad Agraria del Peru, La Molina, Lima 12, Perú 2Bioversity International, Parc Scientifique Agropolis II, Montpellier, 34397, France 3Alliance of Bioversity International and CIAT, Cali, 763537, Colombia 4Instituto Nacional de Innovación Agraria, Lima, 2791, Peru Published Online:29 Jun 2022https://doi.org/10.1094/PDIS-09-21-1951-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleFusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4), the causal agent of Fusarium wilt of banana (FWB), is currently the major threat to the banana industry worldwide (Dita et al. 2018). Restricted to South Asia for more than 20 years, Foc TR4 has spread in the last years to the Middle East, Mozambique, and Colombia (García-Bastidas et al. 2020; https://pestdisplace.org/embed/news/map/disease/11). The incursion of Foc TR4 in Colombia increased awareness and prevention efforts across Latin America and the Caribbean (LAC). However, new Foc TR4 outbreaks in LAC countries were considered inevitable. In April 2021, banana (Musa spp., Cavendish, AAA) plants (30% of incidence) showing typical symptoms of FWB, such as leaf yellowing, wilting, and vascular discoloration, were observed in one farm (about 1 ha) located in Querecotillo, Peru (4°43′54.84″ S, 80°33′45.00″ W). Mycological analyses of samples (pseudostem strands) collected from 10 symptomatic plants were performed as described by Dita et al. (2010). These analyses revealed a continuous presence of fungal colonies identified as F. oxysporum species complex. Molecular diagnostics targeting two different genome regions (Dita et al. 2010; Li et al. 2013) identified nine of these isolates as Foc TR4. These results were further confirmed by qPCR analyses using the commercial Clear Detections TR4 kit. The genomes of four single-spore isolates (PerS1, PerS2, PerS3, and PerS4) were sequenced using the Illumina platform (MiSeq Kit, 2x151 bp Paired-End). The strain PerS4 was also sequenced using Oxford Nanopore (FLOW-MIN111; R10.3 chemistry) as described by López-Alvarez et al. (2020). The generated draft assembly yielded 533 contigs for a size of 47 Mbp (BioProject: PRJNA755905), which is comparable with sizes of previously reported Foc TR4 strains (Asai et al. 2019; García-Bastidas et al. 2020; Maymon et al. 2020; Warmington et al. 2019; Zheng et al. 2018). The sequence assembly showed high contiguity (94.9%) and high similarity (95.48%) with the high-quality genome sequence of the Foc TR4 isolate 'UK0001' (Warmington et al. 2019). Further analyses to identify the presence/absence of full sequences for the putative effector genes (Secreted in Xylem - SIX) and their allelic copies also revealed that the SIX gene profiles of the strains isolated from Querecotillo matched with previously reported Foc TR4 isolates (Czislowski et al. 2017). Pathogenicity tests with three isolates and water controls were performed as described by Dita et al. (2010), using five Cavendish plantlets per treatment. Four weeks after the inoculation, typical external and internal symptoms of FWB were observed only in the inoculated plants. Fungal isolates recovered from inoculated plants tested positive for Foc TR4 when analyzed with PCR diagnostics as mentioned above. No fungal isolates were recovered from water-control plants, which did not show any symptoms. Altogether, our results confirm the first incursion of Foc TR4 in Peru. Currently, Foc TR4 has the phytosanitary status of a present pest with restricted distribution in Peru, and it is under official control of the National Plant Protection Organization – SENASA. Reinforced prevention and quarantine measures, disease monitoring, and capacity building to detect, contain and manage eventual new outbreaks of Foc TR4 are strongly encouraged across LAC banana-producing countries, especially those bordering Peru with larger banana plantations, such as Ecuador and Brazil.The author(s) declare no conflict of interest.References:Asai, S., et al. 2019. Microbiol. Resour. Announc. 8:654. https://doi.org/10.1128/MRA.00654-19Crossref, ISI, Google ScholarCzislowski, E., et al. 2017. Mol. Plant Pathol. 19:1155. https://doi.org/10.1111/mpp.12594Crossref, ISI, Google ScholarDita, M., et al. 2010. Plant Pathol. 59:348. https://doi.org/10.1111/j.1365-3059.2009.02221.xCrossref, ISI, Google ScholarDita, M., et al. 2018. Front. Plant Sci. 9:1468. https://doi.org/10.3389/fpls.2018.01468Crossref, ISI, Google ScholarGarcía-Bastidas, F. A., et al. 2020. Plant Dis. 104:994. https://doi.org/10.1094/PDIS-09-19-1922-PDNLink, ISI, Google ScholarLi, M., et al. 2013. Can. J. Plant Pathol. 35:482. https://doi.org/10.1080/07060661.2013.828321Crossref, Google ScholarLópez-Alvarez, D., et al. 2020. Microbiol. Resour. Announc. 9:e00448. https://doi.org/10.1128/MRA.00448-20Google ScholarMaymon, M., et al. 2020. Sci. Rep. 10:1590. https://doi.org/10.1038/s41598-020-58378-9Crossref, ISI, Google ScholarWarmington, R. J., et al. 2019. Microbiol. Resour. Announc. 8:e00904. https://doi.org/10.1128/MRA.00904-19Crossref, ISI, Google ScholarZheng, S.-J., et al. 2018. Front. Plant Sci. 9:457. https://doi.org/10.3389/fpls.2018.00457Crossref, ISI, Google ScholarFunding: This work was supported by grants from the STC-CGIAR, MIDAGRI, Peru (A1422), the CGIAR Research Program on Roots Tubers and Bananas (RTB), and Consortium of International Agricultural Research Centers. The authors thank GenLab del Perú S.A.C for supporting the whole-genome sequencing of fungal strains.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 8 August 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Cover Image Metrics Article History Issue Date: 29 Jul 2022Published: 29 Jun 2022First Look: 17 Dec 2021Accepted: 6 Dec 2021 Page: 2268 Information© 2022 The American Phytopathological SocietyFundingSTC-CGIAR, MIDAGRI, PeruGrant/Award Number: A1422Consortium of International Agricultural Research CentersKeywordsbananaFusarium oxysporum f. sp. cubenseTropical Race 4The author(s) declare no conflict of interest.PDF downloadCited byBehavior and Use of Quaternary Ammonium-Based Disinfectants in Biosafety Protocols Against Fusarium oxysporum f. sp. cubense Race 1 and Tropical Race 4Laura Arango-Palacio, Andrés M. Pinzón-Núñez, Lilliana Hoyos-Carvajal, Daniel F. Ospina-Galeano, Diego F. Feria-Gómez, Luisa F. Izquierdo-García, Mónica Betancourt-Vásquez, and Sebastián Zapata-Henao29 March 2024 | Plant Disease, Vol. 0, No. 0The Vulnerability of Cuban Banana Production to Fusarium Wilt Caused by Tropical Race 4Einar Martínez de la Parte, Luis Pérez-Vicente, Fernando García-Bastidas, Idalmis Bermúdez-Caraballoso, Sabine Schnabel, Harold J. G. Meijer, and Gert H. J. Kema14 February 2024 | Phytopathology®, Vol. 114, No. 1Disentangling the resistant mechanism of Fusarium wilt TR4 interactions with different cultivars and its elicitor application2 March 2023 | Frontiers in Plant Science, Vol. 14Dissemination of Fusarium Wilt of Banana in Mozambique Caused by Fusarium odoratissimum Tropical Race 4Anouk C. van Westerhoven, Harold J. G. Meijer, Joost Houdijk, Einar Martínez de la Parte, Elie Luntadila Matabuana, Michael F. Seidl, and Gert H. J. Kema20 March 2023 | Plant Disease, Vol. 107, No. 3The Population of Fusarium oxysporum f. sp. cubense in Brazil Is Not Structured by Vegetative Compatibility Group or by Geographic OriginIzabel C. A. Batista, Daniel W. Heck, Alessandro Santos, Gabriel Alves, Camila G. Ferro, Miguel Dita, Fernando Haddad, Sami J. Michereff, Kamilla C. Correia, Christiana F. B. da Silva, and Eduardo S. G. Mizubuti30 November 2022 | Phytopathology®, Vol. 112, No. 11Draft Genome Sequence of Fusarium oxysporum f. sp. cubense Tropical Race 4 from Peru, Obtained by Nanopore and Illumina Hybrid AssemblyMicrobiology Resource Announcements, Vol. 11, No. 9Molecular, Histological and Histochemical Responses of Banana Cultivars Challenged with Fusarium oxysporum f. sp. cubense with Different Levels of Virulence7 September 2022 | Plants, Vol. 11, No. 18
Referência(s)