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Monocytes contribute to a pro‐healing response in 40 μm diameter uniform‐pore, precision‐templated scaffolds

2021; Wiley; Volume: 16; Issue: 3 Linguagem: Inglês

10.1002/term.3280

1932-7005

Nathan R. Chan, Billanna Hwang, Buddy D. Ratner, James D. Bryers,

Wound Healing and Treatments

Journal of Tissue Engineering and Regenerative MedicineVolume 16, Issue 3 p. 297-310 RESEARCH ARTICLE Monocytes contribute to a pro-healing response in 40 μm diameter uniform-pore, precision-templated scaffolds Nathan R. Chan, Nathan R. Chan orcid.org/0000-0003-1809-5186 Molecular Engineering and Sciences Institute, University of Washington, Seattle, Washington, USA Department of Bioengineering, University of Washington, Seattle, Washington, USASearch for more papers by this authorBillanna Hwang, Billanna Hwang Center for Lung Biology, University of Washington, Seattle, Washington, USA Department of Surgery, University of Washington, Seattle, Washington, USASearch for more papers by this authorBuddy D. Ratner, Buddy D. Ratner Molecular Engineering and Sciences Institute, University of Washington, Seattle, Washington, USA Department of Bioengineering, University of Washington, Seattle, Washington, USA Department of Chemical Engineering, University of Washington, Seattle, Washington, USASearch for more papers by this authorJames D. Bryers, Corresponding Author James D. Bryers jbryers@uw.edu orcid.org/0000-0003-3332-3271 Molecular Engineering and Sciences Institute, University of Washington, Seattle, Washington, USA Department of Bioengineering, University of Washington, Seattle, Washington, USA Correspondence James D. Bryers, 3720 15th Ave NE, Seattle, WA 98105, USA. Email: jbryers@uw.eduSearch for more papers by this author Nathan R. Chan, Nathan R. Chan orcid.org/0000-0003-1809-5186 Molecular Engineering and Sciences Institute, University of Washington, Seattle, Washington, USA Department of Bioengineering, University of Washington, Seattle, Washington, USASearch for more papers by this authorBillanna Hwang, Billanna Hwang Center for Lung Biology, University of Washington, Seattle, Washington, USA Department of Surgery, University of Washington, Seattle, Washington, USASearch for more papers by this authorBuddy D. Ratner, Buddy D. Ratner Molecular Engineering and Sciences Institute, University of Washington, Seattle, Washington, USA Department of Bioengineering, University of Washington, Seattle, Washington, USA Department of Chemical Engineering, University of Washington, Seattle, Washington, USASearch for more papers by this authorJames D. Bryers, Corresponding Author James D. Bryers jbryers@uw.edu orcid.org/0000-0003-3332-3271 Molecular Engineering and Sciences Institute, University of Washington, Seattle, Washington, USA Department of Bioengineering, University of Washington, Seattle, Washington, USA Correspondence James D. Bryers, 3720 15th Ave NE, Seattle, WA 98105, USA. Email: jbryers@uw.eduSearch for more papers by this author First published: 29 December 2021 https://doi.org/10.1002/term.3280Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Porous precision-templated scaffolds (PTS) with uniformly distributed 40 μm spherical pores have shown a remarkable ability in immunomodulating resident cells for tissue regeneration. While the pore size mediated pro-healing response observed only in 40 μm pore PTS has been attributed to selective macrophage polarization, monocyte recruitment and phenotype have largely been uncharacterized in regulating implant outcome. Here, we employ a double transgenic mouse model for myeloid characterization and a multifaceted phenotyping approach to quantify monocyte dynamics within subcutaneously implanted PTS. Within 40 μm PTS, myeloid cells were found to preferentially infiltrate into the scaffold. Additionally, macrophage receptor with collagenous structure (MARCO), an innate activation marker, was significantly upregulated within 40 μm PTS. When 40 μm PTS were implanted in monocyte-depleted mice, the transcription of MARCO was significantly decreased and an increase in pro-inflammatory inducible nitric oxide synthase (iNOS) and tumor necrosis factor alpha (TNFα) were observed. Typical of a foreign body response (FBR), 100 μm PTS significantly upregulated pro-inflammatory iNOS, secreted higher amounts of TNFα, and displayed a pore size dependent morphology compared to 40 μm PTS. Overall, these results identify a pore size dependent modulation of circulating monocytes and implicates MARCO expression as a defining subset of monocytes that appears to be responsible for regulating a pro-healing host response. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available from the corresponding author upon reasonable request. Supporting Information Filename Description term3280-sup-0001-suppl-data.docx2.5 MB Supporting Information S1 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume16, Issue3March 2022Pages 297-310 RelatedInformation