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Protocol for cortical-wide field-of-view two-photon imaging with quick neonatal adeno-associated virus injection

2021; Elsevier BV; Volume: 2; Issue: 4 Linguagem: Inglês

10.1016/j.xpro.2021.101007

2666-1667

Ikumi Oomoto, Hiroyuki Uwamori, Chie Matsubara, Maya Odagawa, Midori Kobayashi, Kenta Kobayashi, Keisuke Ota, Masanori Murayama,

interferon and immune responses

We recently established a simple and versatile adeno-associated virus (AAV) induction approach that enables dense (>90% labeled neurons) and cortical-wide Ca2+ sensor expression. Here, we describe the stepwise protocol for neonatal AAV injection of a Ca2+ sensor. We also detail the steps for subsequent craniotomy to generate a chronic cranial window, followed by wide-field two-photon Ca2+ imaging in an awake mouse. This protocol serves as an alternative to the use of transgenic animals and offers translatable options for cortical-wide experiments. For complete details on the use and execution of this protocol, please refer to Ota et al. (2021).