Produção Nacional
Revisado por pares
Toxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health?
2021; Elsevier BV; Volume: 813; Linguagem: Inglês
10.1016/j.scitotenv.2021.152345
ISSN1879-1026
AutoresBianca Helena Ventura Fernandes, Natália Martins Feitosa, Ana Paula Barbosa, Camila Gasque Bomfim, Anali M. B. Garnique, Ivana F. Rosa, Maira da Silva Rodrigues, Lucas B. Doretto, Daniel Fernandes da Costa, Bruno Camargo-Dos-Santos, Gabrielli A. Franco, João Favero Neto, Juliana Sartori Lunardi, Marina Bellot, Nina Pacheco Capelini Alves, Camila Carlino da Costa, Mayumi Fernanda Aracati, Letícia Franchin Rodrigues, Camila Carlino da Costa, Rafaela Hemily Cirilo, Raul Marcelino Colagrande, Francisco Isaac Fernandes Gomes, Rafael Takahiro Nakajima, Marco Antônio de Andrade Belo, Percília Cardoso Giaquinto, Susana Luporini de Oliveira, Silas Fernandes Eto, Dayanne Carla Fernandes, Wilson Gómez Manrique, Gabriel Conde, Roberta Costa, Íris Todeschini, Ilo Rivero, Edgar E. Llontop, Germán G. Sgro, Gabriel Umaji Oka, Natalia Fernanda Bueno, F Ferraris, Mariana T. Q. de Magalhães, Renata Jurema Medeiros, Juliana Moreira Mendonça-Gomes, Mara de Souza Junqueira, Kátia Conceição, Letícia Gomes de Pontes, Antônio Condino‐Neto, Andrea Perez, Leonardo José Gil Barcellos, José Dias Corrêa, Erick Gustavo Dorlass, Niels Olsen Saraiva Câmara, Edison Luíz Durigon, Fernando Q. Cunha, Rafael Henrique Nóbrega, Gláucia Maria Machado‐Santelli, Chuck S. Farah, Flávio P. Veras, Jorge Galindo‐Villegas, Letícia V. Costa‐Lotufo, Thiago M. Cunha, Roger Chammas, Luciani R. Carvalho, Cristiane Rodrigues Guzzo, Guilherme Malafaia, Ives Charlie‐Silva,
Tópico(s)SARS-CoV-2 detection and testing
ResumoDespite the significant increase in the generation of SARS-CoV-2 contaminated domestic and hospital wastewater, little is known about the ecotoxicological effects of the virus or its structural components in freshwater vertebrates. In this context, this study evaluated the deleterious effects caused by SARS-CoV-2 Spike protein on the health of Danio rerio, zebrafish. We demonstrated, for the first time, that zebrafish injected with fragment 16 to 165 (rSpike), which corresponds to the N-terminal portion of the protein, presented mortalities and adverse effects on liver, kidney, ovary and brain tissues. The conserved genetic homology between zebrafish and humans might be one of the reasons for the intense toxic effects followed inflammatory reaction from the immune system of zebrafish to rSpike which provoked damage to organs in a similar pattern as happen in severe cases of COVID-19 in humans, and, resulted in 78,6% of survival rate in female adults during the first seven days. The application of spike protein in zebrafish was highly toxic that is suitable for future studies to gather valuable information about ecotoxicological impacts, as well as vaccine responses and therapeutic approaches in human medicine. Therefore, besides representing an important tool to assess the harmful effects of SARS-CoV-2 in the aquatic environment, we present the zebrafish as an animal model for translational COVID-19 research.
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