Portal de Periódicos da CAPES

Ultra-Deep Sequencing of Plasma-Circulating DNA for the Detection of Tumor- Derived Mutations in Patients with Nonmetastatic Colorectal Cancer

2021; Taylor & Francis; Volume: 40; Issue: 4 Linguagem: Inglês

10.1080/07357907.2021.2017951

1532-4192

Huu Thinh Nguyen, Bac An Luong, Duc Huy Tran, Trong-Hieu Nguyen, Quoc Dat Ngo, Linh Gia Hoang Le, Quoc Ho, Hue-Hanh Thi Nguyen, Cao Minh Nguyen, Vu Uyen Tran, Truong Vinh Ngoc Pham, Minh Triết Lê, Ngoc An Trinh Le, Trung Kien Le, Nguyễn Thành Luân, Hong-Anh Thi Pham, Hong Thuy Le, Duong Thi Hong Diep, Anh Vu Hoang, Nguyen Hoang Bac, Kiet Truong Dinh, Minh‐Duy Phan, Hoai‐Nghia Nguyen, Thanh‐Thuy Thi, Hoa Giang, Le Son Tran, Tuan Diep Tran,

Lung Cancer Treatments and Mutations

Identification of tumor-derived mutation (TDM) in liquid biopsies (LB), especially in early-stage patients, faces several challenges, including low variant-allele frequencies, interference by white blood cell (WBC)-derived mutations (WDM), benign somatic mutations and tumor heterogeneity. Here, we addressed the above-mentioned challenges in a cohort of 50 nonmetastatic colorectal cancer patients, via a workflow involving parallel sequencing of paired WBC- and tumor-gDNA. After excluding potential false positive mutations, we detected at least one TDM in LB of 56% (28/50) of patients, with the majority showing low-patient coverage, except for one TDM mapped to KMT2D that recurred in 30% (15/30) of patients.