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PCR performance in the SARS-CoV-2 Omicron variant of concern?

2021; EMH Swiss Medical Publishers Ltd.; Volume: 151; Issue: 4950 Linguagem: Inglês

10.4414/smw.2021.w30120

0036-7672

César Metzger, Reto Lienhard, Helena M. B. Seth-Smith, Tim Roloff, Fanny Wegner, Jonas Sieber, Michael Bel, Gilbert Greub, Adrian Egli,

Animal Virus Infections Studies

The new SARS-CoV-2 Omicron variant (B.1.1.529) has been recently declared a Variant of Concern due to a series of important mutations in the viral spike protein and especially in the receptor-binding domain. While investigations into the spread of this new variant are ongoing, the first cases have been detected in Switzerland. Important questions have been raised: (1) Will the PCR assays commonly used to detect SARS-CoV-2 still work for the Omicron variant? (2) Can specific PCR features, e.g. S-gene dropout, be used to identify potential Omicron samples? In this minireview we provide current knowledge on the Omicron variant and guidance on its PCR validation.