Produção Nacional
Toxoplasma gondii in tissues of sheep slaughtered for human consumption in a highly endemic area for toxoplasmosis in Brazil
2022; Elsevier BV; Volume: 29; Linguagem: Inglês
10.1016/j.vprsr.2022.100688
ISSN2405-9390
AutoresA.F. Silva, Edwards Frazão-Teixeira, Francisco Carlos Rodrigues de Oliveira, Ana Beatriz Monteiro Fonseca, Ana Maria Reis Ferreira,
Tópico(s)Herpesvirus Infections and Treatments
ResumoToxoplasma gondii infects warm-blooded animals, including humans, and the ingestion of undercooked meat of infected animals is an important source of infection. We investigated the presence of T. gondii by PCR and histopathology in tissues of 25 seropositive sheep slaughtered for human consumption in Rio de Janeiro and assessed the suitability of different tissues for molecular detection of the parasite. The animals were first screened for T. gondii antibodies by MAT (Modified Agglutination Test), and tissues of seropositive sheep (brain, heart, lungs, kidneys, liver and diaphragm) were subjected to molecular and histological examination. A nested-PCR targeting the P43 gene of T. gondii was performed, and kappa Coefficient was used to assess PCR results among tissues. DNA of the parasite was detected in 60 % (9 of 15) of the animals. and tissue pairs of lungs/heart, lungs/diaphragm or heart/diaphragm had substantial agreement with the global status of the animals. The combination between these three tissues leads to an almost perfect agreement with global status results. Sheep slaughtered for human consumption in Rio de Janeiro, Brazil are infected with T. gondii, and pairs of fragments of lungs and heart, lungs and diaphragm or heart and diaphragm could be used for molecular identification of T. gondii in sheep with substantial agreement with the global status of the animals.
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