First Report of Fruit Rot Caused by Fusarium luffae in Muskmelon in China
2022; American Phytopathological Society; Volume: 106; Issue: 6 Linguagem: Inglês
10.1094/pdis-12-21-2666-pdn
ISSN1943-7692
AutoresX. P. Zhang, Xiaodong Cao, Q. Q. Dang, Y. G. Liu, Xiaoping Zhu, Ji-Wen Xia,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 106, No. 6First Report of Fruit Rot Caused by Fusarium luffae in Muskmelon in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Fruit Rot Caused by Fusarium luffae in Muskmelon in ChinaX. P. Zhang, X. D. Cao, Q. Q. Dang, Y. G. Liu, X. P. Zhu, and J. W. XiaX. P. ZhangShandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China, X. D. CaoYinan County Agricultural and Rural Bureau, Linyi, Shandong, 276300, China, Q. Q. DangYutai County Bureau of Planning and Natural Resources, Jining, Shandong, 272000, China, Y. G. LiuShandong Provincial University Laboratory for Protected Horticulture, Weifang University of Science and Technology, Weifang, Shandong, 262700, China, X. P. Zhu†Corresponding authors: X. P. Zhu; E-mail Address: zhuxp@sdau.edu.cn, and J. W. Xia; E-mail Address: xiajiwen1@126.comhttps://orcid.org/0000-0001-7236-731XShandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China, and J. W. Xia†Corresponding authors: X. P. Zhu; E-mail Address: zhuxp@sdau.edu.cn, and J. W. Xia; E-mail Address: xiajiwen1@126.comhttps://orcid.org/0000-0002-7436-7249Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China AffiliationsAuthors and Affiliations X. P. Zhang1 X. D. Cao2 Q. Q. Dang3 Y. G. Liu4 X. P. Zhu1 † J. W. Xia1 † 1Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China 2Yinan County Agricultural and Rural Bureau, Linyi, Shandong, 276300, China 3Yutai County Bureau of Planning and Natural Resources, Jining, Shandong, 272000, China 4Shandong Provincial University Laboratory for Protected Horticulture, Weifang University of Science and Technology, Weifang, Shandong, 262700, China Published Online:12 May 2022https://doi.org/10.1094/PDIS-12-21-2666-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleMuskmelon (Cucumis melo L.) is one of the most widely cultivated and economically important fruit crops in the world. However, many pathogens can cause decay of muskmelon fruit, including Fusarium spp. Fusarium spp. are the most important pathogen, affecting muskmelon fruit yield and quality (Wang et al. 2011). In August 2020, fruit rot symptoms were observed on ripening muskmelons (cv. Tianbao) in several fields in Jiyang District, Jinan City of Shandong Province, China. The incidences of infected muskmelon ranged from 15 to 30% and caused an average 20% yield loss. Symptoms appeared as pale brown, water-soaked lesions that were irregular in shape, with the lesion sizes ranging from a small spot (1 to 2 cm) to decay of the entire fruit. The core and surface of infected fruit were colonized and covered with white mycelia. Two infected muskmelons were collected from two fields, 3.5 km apart. Tissues removed from inside the infected fruit were surface disinfected with 75% ethanol for 30 s, and cultured on potato dextrose agar (PDA) at 25°C in the dark for 5 days. Four purified cultures were obtained using the single spore method. On carnation leaf agar (CLA), 3 to 5 septate, falcate, with a pronounced dorsiventral curvature macroconidia with tapered apical cell, and foot-shaped basal cell, measuring 20 to 40 × 3.5 to 4.5 μm. Microconidia and chlamydospores were not observed. These morphological characteristics were consistent with the description of F. luffae (Wang et al. 2019). Because these isolates had similar morphology, two representative isolates (XP11 and XP12) were selected for multilocus phylogenetic analyses. DNA was extracted from the representative isolates using a CTAB method. Nucleotide sequences of the internal transcribed spacers (ITS) (White et al. 1990), calmodulin (CAM), RNA polymerase II second largest subunit (RPB2), and translation elongation factor 1-α gene (TEF1) (Xia et al. 2019) were amplified using specific primers, sequenced, and deposited in GenBank (ITS: MW391509 and MW391510, CAM: MW392789 and MW392790, RPB2: MW392797 and MW392798, TEF1: MW392793 and MW392794). Alignments of a combined dataset of ITS, CAM, RPB2, and TEF1 were made using MAFFT v. 7, and phylogenetic analyses were conducted in MEGA v. 7.0 using the maximum likelihood method. The muskmelon isolates (XP11 and XP12) clustered together with the F. luffae reference strain LC12167 (99% bootstrap). To perform a pathogenicity test, 10 μl of conidial suspensions (1 × 106 conidia/ml) were injected into each muskmelon fruit using a syringe, and the control fruit was inoculated with 10 μl of sterile distilled water. There were 10 replicated fruits for each treatment. The test was repeated three times. After 7 days at 25°C, the interior of the inoculated muskmelons begun to rot, and the rot lesion expanded from the core toward the surface of the fruit, then white mycelia were produced on the surface. Ten isolations were reisolated from the infected tissues and confirmed to fulfill Koch's postulates. No symptoms were observed on the control muskmelons. To our knowledge, this is the first report of fruit rot caused by F. luffae in muskmelon in China. Considering the economic value of the muskmelon crop, correct identification can help farmers select appropriate field management measures for control of this disease.The author(s) declare no conflict of interest.References:Wang, J., et al. 2011. J. Agric. Food Chem. 59:12527. https://doi.org/10.1021/jf203775q Crossref, ISI, Google ScholarWang, M. M., et al. 2019. Persoonia 43:70. https://doi.org/10.3767/persoonia.2019.43.03 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Google ScholarXia, J. W., et al. 2019. Persoonia 43:186. https://doi.org/10.3767/persoonia.2019.43.05 Crossref, ISI, Google ScholarFunding: This study was funded by Shandong Provincial Agricultural Major Applied Technology Innovation Project (grant no. SD2019ZZ004), Key Research and Development Program of Shandong Province (2019JZZY010707), and Weifang Science and Technology Development (2020GX062).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 106, No. 6 June 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 2 Jun 2022Published: 12 May 2022First Look: 3 Jan 2022Accepted: 30 Dec 2021 Page: 1763 Information© 2022 The American Phytopathological SocietyFundingShandong Provincial Agricultural Major Applied Technology Innovation ProjectGrant/Award Number: SD2019ZZ004Key Research and Development Program of Shandong ProvinceGrant/Award Number: 2019JZZY010707Weifang Science and Technology DevelopmentGrant/Award Number: 2020GX062Keywordsfruit rotFusarium luffaemuskmelonThe author(s) declare no conflict of interest.PDF download
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