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BIBTEX RIS

TcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens

2022; Nature Portfolio; Volume: 12; Issue: 1 Linguagem: Inglês

10.1038/s41598-021-04700-y

ISSN

2045-2322

Autores

Milena do Amaral, Ana Camila Oliveira Freitas, Ariana Silva Santos, Everton Cruz dos Santos, Monaliza Macêdo Ferreira, Abelmon da Silva Gesteira, Karina Peres Gramacho, Jeanne Scardini Marinho‐Prado, Carlos Priminho Pirovani,

Tópico(s)

Food Chemistry and Fat Analysis

Resumo

Protease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10

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