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Correction: Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus

2012; Public Library of Science; Volume: 7; Issue: 8 Linguagem: Inglês

10.1371/annotation/2965cfae-b77d-4014-8b7b-236e01a35492

1932-6203

Chien-Te Tseng, Elena Sbrana, Naoko Iwata‐Yoshikawa, Patrick C. Newman, Tania Garron, Robert L. Atmar, C. J. Peters, Robert B. Couch,

Viral gastroenteritis research and epidemiology

Background: Severe acute respiratory syndrome (SARS) emerged in China in 2002 and spread to other countries before brought under control.Because of a concern for reemergence or a deliberate release of the SARS coronavirus, vaccine development was initiated.Evaluations of an inactivated whole virus vaccine in ferrets and nonhuman primates and a viruslike-particle vaccine in mice induced protection against infection but challenged animals exhibited an immunopathologictype lung disease.Design: Four candidate vaccines for humans with or without alum adjuvant were evaluated in a mouse model of SARS, a VLP vaccine, the vaccine given to ferrets and NHP, another whole virus vaccine and an rDNA-produced S protein.Balb/c or C57BL/6 mice were vaccinated IM on day 0 and 28 and sacrificed for serum antibody measurements or challenged with live virus on day 56.On day 58, challenged mice were sacrificed and lungs obtained for virus and histopathology.Results: All vaccines induced serum neutralizing antibody with increasing dosages and/or alum significantly increasing responses.Significant reductions of SARS-CoV two days after challenge was seen for all vaccines and prior live SARS-CoV.All mice exhibited histopathologic changes in lungs two days after challenge including all animals vaccinated (Balb/C and C57BL/6) or given live virus, influenza vaccine, or PBS suggesting infection occurred in all.Histopathology seen in animals given one of the SARS-CoV vaccines was uniformly a Th2-type immunopathology with prominent eosinophil infiltration, confirmed with special eosinophil stains.The pathologic changes seen in all control groups lacked the eosinophil prominence.Conclusions: These SARS-CoV vaccines all induced antibody and protection against infection with SARS-CoV.However, challenge of mice given any of the vaccines led to occurrence of Th2-type immunopathology suggesting hypersensitivity to SARS-CoV components was induced.Caution in proceeding to application of a SARS-CoV vaccine in humans is indicated.