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Negeviruses isolated from mosquitoes in the Brazilian Amazon

2022; BioMed Central; Volume: 19; Issue: 1 Linguagem: Inglês

10.1186/s12985-022-01743-z

1743-422X

Ana Cláudia da Silva Ribeiro, Livia Carício Martins, Sandro Patroca da Silva, Daniele Barbosa de Almeida Medeiros, Keissy Karoline Pinheiro Miranda, Joaquim Pinto Nunes Neto, Hamilton Antônio de Oliveira Monteiro, Bruna Laís Sena do Nascimento, José Wilson Rosa, Ana Cecília Ribeiro Cruz, Pedro Fernando da Costa Vasconcelos, Valéria Lima Carvalho, Sueli Guerreiro Rodrigues,

Insect-Plant Interactions and Control

Abstract Background There are several groups of viruses including Insect Specific Viruses (ISV) such as the taxon Negevirus , a group of viruses phylogenetically related to plant viruses. Negeviruses replicate in mosquito cells, but not in vertebrate cells. Methods Pools of hematophagous arthropods were inoculated in Vero and C6/36 cells. The cells were observed to detect possible cytopathic effect. Then, indirect immunofluorescence, RT-PCR, and nucleotide sequencing were performed. Results Seven samples which presented negative results for flaviviruses, alphaviruses and bunyaviruses, but showed cytopathic effect in C6/36 cells were sequenced. We identified the occurrence of a variety of ISVs, most of them belonging to the taxon Negevirus : The Brejeira, Negev, Cordoba and Wallerfield viruses, including a new virus for science, tentatively named Feitosa virus. Conclusions We detected negeviruses in the Amazon region, including two viruses that were isolated for the first time in Brazil: Cordoba virus and the Negev virus and, a new virus for science: the Feitosa virus.