Antigen-Antibody Reactions
2014; Linguagem: Inglês
10.1128/9781555817497.ch8
AutoresLucy M. Mutharia, Joseph S. Lam,
Tópico(s)Immunodeficiency and Autoimmune Disorders
ResumoChapter 8 Antigen-Antibody Reactions Lucy M. Mutharia, Lucy M. MuthariaSearch for more papers by this authorJoseph S. Lam, Joseph S. LamSearch for more papers by this author Lucy M. Mutharia, Lucy M. MuthariaSearch for more papers by this authorJoseph S. Lam, Joseph S. LamSearch for more papers by this author Book Editor(s):C. A. Reddy, C. A. ReddySearch for more papers by this authorT. J. Beveridge, T. J. BeveridgeSearch for more papers by this authorJ. A. Breznak, J. A. BreznakSearch for more papers by this authorG. A. Marzluf, G. A. MarzlufSearch for more papers by this authorT. M. Schmidt, T. M. SchmidtSearch for more papers by this authorL. R. Snyder, L. R. SnyderSearch for more papers by this author First published: 17 August 2007 https://doi.org/10.1128/9781555817497.ch8 AboutPDFPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShareShare a linkShare onFacebookTwitterLinked InRedditWechat Summary Reactions between antigens (Ags) and antibodies (Abs) are usefully exploited in many areas of life science research. The monoclonal Ab (MAb) technology developed by Köhler and Milstein allows for the production of unlimited quantities of Abs against virtually any molecule. As there is an immense volume of information concerning all aspects of Ag-Ab reactions in the literature, the objective of this chapter is to provide simple and useful protocols and an introduction to some of the more novel techniques. The routes of injection commonly used on rabbits, mice and rats include intradermal (i.d.), subcutaneous (s.c.), intramuscular (i.m.), intraperitoneal (i.p.), and intravenous (i.v.). Nucleic acid (DNA or RNA) immunization and genetic vaccines introduced in the early 1990s are some of the most important discoveries and novel strategies in vaccine development. The essential features of a DNA vaccine are a bacterial plasmid vector engineered to carry a DNA insert encoding the protein immunogen(s) of interest, a eukaryote gene promoter, and a poly(A) site to enable expression of the protein in mammalian cells. The vectors are usually maintained in and purified from Escherichia coli. The polyclonal serum is expressed from the blood clot by centrifugation, and approximately 52% of its volume can be collected as serum. The serum can be used directly in Ag-Ab reactions, absorbed to deplete it of nonspecific or cross-reactive Abs, or fractionated to purify Abs free of other serum proteins. Methods for General and Molecular Microbiology, 3rd Edition RelatedInformation
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