Portal de Periódicos da CAPES

Transgenic Animals

1989; Elsevier BV; Linguagem: Inglês

10.1016/s0074-7696(08)60630-0

2163-5854

Jon W. Gordon,

Virus-based gene therapy research

This chapter describes the mechanisms of foreign DNA integration in transgenic mice. The fundamentals of gene regulation revealed by transgenic mice are discussed. The application of principles of gene regulation to the study of specific problems including immune-system regulation, oncogenesis, creation of animal models for human disease, development of models for gene therapy, ablation of specific cell lineages in animals, studies of X-chromosome inactivation, and investigations of the effects of parental legacy on gene expression are described. Some observations on transgenic mice are reviewed. The potential for creating mutations by foreign gene insertion is also presented. Improved techniques for constructing chimeras and the development of recombinant DNA and gene transfer technologies have created the potential for inserting single cloned genes into the germ lines of mice. Teratocarcinomas—malignancies derived from early embryo cells—are developmentally normalized after insertion into recipient blastocysts. Teratocarcinoma cells are maintained in culture for prolonged periods and after microinjection into the blastocoel, cooperate in the production of normally differentiated organs of chimeras.