Optimization of the SARS-CoV-2 ARTIC Network V4 Primers and Whole Genome Sequencing Protocol
2022; Frontiers Media; Volume: 9; Linguagem: Inglês
10.3389/fmed.2022.836728
ISSN2296-858X
AutoresArnold W. Lambisia, Khadija Said Mohammed, Timothy Makori, Leonard Ndwiga, Maureen W. Mburu, John Mwita Morobe, Edidah O. Moraa, Jennifer Musyoki, Nickson Murunga, Jane N. Mwangi, D. James Nokes, Charles N. Agoti, Lynette Isabella Ochola‐Oyier, George Githinji,
Tópico(s)Plant Virus Research Studies
ResumoThe ARTIC Network's primer set and amplicon-based protocol is one of the most widely used SARS-CoV-2 sequencing protocol. An update to the V3 primer set was released on 18th June 2021 to address amplicon drop-off observed among the Delta variant of concern. Here, we report on an in-house optimization of a modified version of the ARTIC Network V4 protocol that improves SARS-CoV-2 genome recovery in instances where the original V4 pooling strategy was characterized by amplicon drop-offs.
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