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Powdered coconut water with cryoprotectant can be used for cooling and future freezing of Tambaqui ( Colossoma macropomum ) sperm

2022; Wiley; Volume: 38; Issue: 3 Linguagem: Inglês

10.1111/jai.14303

1439-0426

Fátima de Cássia Evangelista de Oliveira, Carminda Sandra Brito Salmito‐Vanderley, Thais Maia Torres, Liliane Veras Leite‐Castro, Cristiane Clemente de Mello Salgueiro, Francisco Renan Aragão Linhares, Maria Audália Marques de Carvalho, Yara Silvino Sales, José Ferreira Nunes,

Aquaculture Nutrition and Growth

The study aims to evaluate the main parameters of extended sperm in a solution of powdered coconut water (ACPTM-104) or glucose, both added or not with cryoprotectants during the following periods of cooling or equilibration time: 0 (time of the dilution), 3, 24, 48, and 72 h. The sperm was maintained at 4°C (cooling temperature) as: un-extended (control) and in six different equilibration media combining two extenders (ACP-104 or Glucose) and two cryoprotectants (methyl glycol or dimethylsulfoxide). After being extended and maintained in different equilibration times, sperm was evaluated for kinetic and morphology parameters. At 0 h, best motility results were found when the sperm was not extended; however, after 48-h of equilibration, the sperm extended in ACPTM-104 (38.24%) showed better motility results than the fresh sperm (24.30%; p < .05). The un-extended, after 72 h of cooling, presented lower motility rate and increased sperm abnormality percentages when compared to the one extended in ACPTM-104 (with or without the addition of cryoprotectants). Thus, tambaqui sperm extended in powdered coconut water can be cooled (4°C) up to 72 h. This study also analyzed tambaqui sperm quality during 72 h of exposure to cryoprotectants, providing data for possible extension of equilibration time when necessary.