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Oviposition deterrence, larvicidal activity and docking of β-germacrene-D-4-ol obtained from leaves of Piper corcovadensis (Piperaceae) against Aedes aegypti

2022; Elsevier BV; Volume: 182; Linguagem: Inglês

10.1016/j.indcrop.2022.114830

1872-633X

Bheatriz Nunes de Lima Albuquerque, Marcelo Felipe Rodrigues Da Silva, Patrícia Cristina Bezerra Da Silva, Camila Soledade de Lira Pimentel, Suyana Karolyne Lino da Rocha, Júlio César Ribeiro de Oliveira Farias de Aguiar, Afonso Cordeiro Agra Neto, Patrícia Maria Guedes Paiva, Matheus Gabriel Moura Gomes, Edeildo Ferreira da Silva‐Júnior, Daniela Maria do Amaral Ferraz Navarro,

Mosquito-borne diseases and control

The present study compared the chromatographic profile of oils from P. corcovadensis leaves obtained through hydrodistillation, maceration, supercritical fluid, headspace, steam distillation and ultrasound to determine the best method for the obtainment of β-germacrene-D-4-ol. The oviposition deterrent and larvicidal activities of this compound were carried out. The main compounds identified in the oils obtained using these methods were terpinolene, 1-butyl-3,4-methylenedioxybenzene, trans-caryophyllene and β-germacrene-D-4-ol, the proportions of which varied with the extraction method employed. Steam distillation (26.48 ± 0.37%), maceration (25.26 ± 0.39%) and supercritical fluid (24.38 ± 0.47%) were the best methods for extracting β-germacrene-D-4-ol. The oil obtained by maceration had the highest yield and was therefore chosen for the isolation of β-germacrene-D-4-ol. Larvicidal and oviposition assays of isolated compound and the oil obtained by maceration were performed with A. aegypti. Larvicidal activity (LC50) were 18.23 ± 1.19 ppm for β-germacrene-D-4-ol and 6.71 ± 0.16 ppm for the oil. The compound and oil also exhibited oviposition deterrence activity against the mosquito at concentrations of 5, 10 and 50 ppm. Moreover, it could be assumed that the sesquiterpene β-germacrene-D-4-ol achieves oviposition deterrence by interacting with odorant-binding protein 1 (OBP1) in Aedes aegypti, as the highest FitScore was found for this macromolecular target. This suggests how mosquito identifies β-germacrene-D-4-ol at the oviposition site. The findings demonstrate that β-germacrene-D-4-ol and the P. corcovadensis leaf oil obtained by maceration have considerable potential as larvicides and oviposition deterrents for the control of the A. aegypti.