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Synergistic Activity of Dobutamine Combined with Azoles and Its Mechanism of Action Against Strains of Candida Glabrata

2022; Future Medicine; Volume: 17; Issue: 6 Linguagem: Inglês

10.2217/fmb-2020-0256

1746-0921

Fátima DD Barroso, Lisandra J da Silva, Lívia Gurgel do Amaral Valente Sá, Cecília Rocha da Silva, João B A de Neto, Francisca BA do Nascimento, Helaine Almeida Queiroz, Amanda C Leitão, Vitória PF Cabral, Daniel Sampaio Rodrigues, Amanda Dias Barbosa, Bruno C. Cavalcanti, Manoel Odorico de Morais, Hélio Vitoriano Nobre Júnior,

Phenothiazines and Benzothiazines Synthesis and Activities

Aims: To evaluate the antifungal effect of dobutamine against Candida glabrata as well as its synergism with azoles and its action on biofilm. Methods: The M27-A3 protocol and flow cytometry were used for elucidation of the possible mechanism of action. Results: The tested isolates presented MICs ranging from 2 to 32 μg/ml for dobutamine, with fungistatic effect. A total of 82% of the strains showed synergism with fluconazole, with 90% showing synergism with itraconazole. The effect on biofilm formation was nonsignificant. Cytometry tests showed that dobutamine induced mitochondrial depolarization. Conclusion: Dobutamine has an antifungal effect on strains of C. glabrata and synergistic activity with azoles. This effect is probably mediated by increased oxidative damage to the membrane.