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Bacillus subtilis spores as delivery system for nasal Plasmodium falciparum circumsporozoite surface protein immunization in a murine model

2022; Nature Portfolio; Volume: 12; Issue: 1 Linguagem: Inglês

10.1038/s41598-022-05344-2

2045-2322

Maria Edilene Martins de Almeida, Késsia Caroline Souza Alves, Maria Gabriella Santos de Vasconcelos, Thiago Serrão Pinto, Juliane Corrêa Glória, Yury Oliveira Chaves, Walter Luiz Lima Neves, Andréa Monteiro Tarragô, Júlio Nino de Souza Neto, Spartaco Astolfi‐Filho, Gemilson Soares Pontes, Antônio Alcirley da Silva Balieiro, Rachele Isticato, Ezio Ricca, Luís André Morais Mariúba,

Monoclonal and Polyclonal Antibodies Research

Abstract Malaria remains a widespread public health problem in tropical and subtropical regions around the world, and there is still no vaccine available for full protection. In recent years, it has been observed that spores of Bacillus subtillis can act as a vaccine carrier and adjuvant, promoting an elevated humoral response after co-administration with antigens either coupled or integrated to their surface. In our study, B. subtillis spores from the KO7 strain were used to couple the recombinant CSP protein of P. falciparum (r Pf CSP), and the nasal humoral-induced immune response in Balb/C mice was evaluated. Our results demonstrate that the spores coupled to r Pf CSP increase the immunogenicity of the antigen, which induces high levels of serum IgG, and with balanced Th1/Th2 immune response, being detected antibodies in serum samples for 250 days. Therefore, the use of B. subtilis spores appears to be promising for use as an adjuvant in a vaccine formulation.