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Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction

2022; Elsevier BV; Volume: 102; Issue: 4 Linguagem: Inglês

10.1016/j.diagmicrobio.2022.115636

1879-0070

Ivaine Taís Sauthier Sartor, Fernanda Hammes Varela, Mariana Rost Meireles, Luciane Beatriz Kern, Thaís Raupp Azevedo, Gabriela Luchiari Tumioto Giannini, Mariana Soares da Silva, Meriane Demoliner, Juliana Schons Gularte, Paula Rodrigues de Almeida, Juliane Deise Fleck, Gabriela Oliveira Zavaglia, Ingrid Rodrigues Fernandes, Caroline Nespolo de David, Amanda Paz Santos, Walquíria Aparecida Ferreira de Almeida, Victor Bertollo Gomes Pôrto, Marcelo Comerlato Scottá, Gustavo Fioravanti Vieira, Fernando Rosado Spilki, Renato T. Stein, Márcia Polese-Bonatto,

vaccines and immunoinformatics approaches

We aimed to describe the SARS-CoV-2 lineages circulating early pandemic among samples with S gene dropout and characterize the receptor-binding domain (RBD) of viral spike protein. Adults and children older than 2 months with signs and symptoms of COVID-19 were prospectively enrolled from May to October in Porto Alegre, Brazil. All participants performed RT-PCR assay, and samples with S gene dropout and cycle threshold < 30 were submitted to high-throughput sequencing (HTS). 484 out of 1,557 participants tested positive for SARS-CoV-2. The S gene dropout was detected in 7.4% (36/484) and a peak was observed in August. The B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic. The RBD novel mutation (Y380Q) was found in one sample occurring simultaneously with C379W and V395A, and the B.1.91 lineage in the spike protein. The Y380Q and C379W may interfere with the binding of neutralizing antibodies (CR3022, EY6A, H014, S304).