The metabolomic profiling identifies N, N‐dimethylglycine as a facilitator of dorsal root ganglia neuron axon regeneration after injury
2022; Wiley; Volume: 36; Issue: 5 Linguagem: Inglês
10.1096/fj.202101698r
ISSN1530-6860
AutoresJun‐Jie Zhang, Chun-Yi Jiang, Xiaohong Liu, Chenxiao Jiang, Qianqian Cao, Bin Yu, Yaohui Ni, Susu Mao,
Tópico(s)Adipose Tissue and Metabolism
ResumoThe FASEB JournalVolume 36, Issue 5 e22305 RESEARCH ARTICLE The metabolomic profiling identifies N, N-dimethylglycine as a facilitator of dorsal root ganglia neuron axon regeneration after injury Junjie Zhang, Junjie Zhang Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaSearch for more papers by this authorChunyi Jiang, Chunyi Jiang Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China Department of Pathology, Affiliated Hospital of Nantong University, Nantong, ChinaSearch for more papers by this authorXiaohong Liu, Xiaohong Liu Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaSearch for more papers by this authorChen-Xiao Jiang, Chen-Xiao Jiang Medical School, Nantong University, Nantong, ChinaSearch for more papers by this authorQianqian Cao, Qianqian Cao Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaSearch for more papers by this authorBin Yu, Bin Yu Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaSearch for more papers by this authorYaohui Ni, Corresponding Author Yaohui Ni niyaohui200688@126.com Department of Neurology, Affiliated Hospital of Nantong University, Nantong, China Correspondence Yaohui Ni, Department of Neurology, Affiliated Hospital of Nantong University, Nantong 226001, China. Email: niyaohui200688@126.com Susu Mao, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. Email: maosusu@ntu.edu.cnSearch for more papers by this authorSusu Mao, Corresponding Author Susu Mao maosusu@ntu.edu.cn Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China Correspondence Yaohui Ni, Department of Neurology, Affiliated Hospital of Nantong University, Nantong 226001, China. Email: niyaohui200688@126.com Susu Mao, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. Email: maosusu@ntu.edu.cnSearch for more papers by this author Junjie Zhang, Junjie Zhang Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaSearch for more papers by this authorChunyi Jiang, Chunyi Jiang Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China Department of Pathology, Affiliated Hospital of Nantong University, Nantong, ChinaSearch for more papers by this authorXiaohong Liu, Xiaohong Liu Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaSearch for more papers by this authorChen-Xiao Jiang, Chen-Xiao Jiang Medical School, Nantong University, Nantong, ChinaSearch for more papers by this authorQianqian Cao, Qianqian Cao Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaSearch for more papers by this authorBin Yu, Bin Yu Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaSearch for more papers by this authorYaohui Ni, Corresponding Author Yaohui Ni niyaohui200688@126.com Department of Neurology, Affiliated Hospital of Nantong University, Nantong, China Correspondence Yaohui Ni, Department of Neurology, Affiliated Hospital of Nantong University, Nantong 226001, China. Email: niyaohui200688@126.com Susu Mao, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. Email: maosusu@ntu.edu.cnSearch for more papers by this authorSusu Mao, Corresponding Author Susu Mao maosusu@ntu.edu.cn Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China Correspondence Yaohui Ni, Department of Neurology, Affiliated Hospital of Nantong University, Nantong 226001, China. Email: niyaohui200688@126.com Susu Mao, Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. Email: maosusu@ntu.edu.cnSearch for more papers by this author First published: 08 April 2022 https://doi.org/10.1096/fj.202101698R Junjie Zhang and Chunyi Jiang contributed equally to this study. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Identifying novel molecules involved in axon regeneration of neurons in the peripheral nervous system (PNS) will be of benefit in obtaining a therapeutic strategy for repairing axon damage both in the PNS and the central nervous system (CNS). Metabolism and axon regeneration are tightly connected. However, the overall metabolic processes and the landscape of the metabolites in axon regeneration of PNS neurons are uncovered. Here, we used an ultra high performance liquid tandem chromatography quadrupole time of flight mass spectrometry (UHPLC-QTOFMS)-based untargeted metabolomics to analyze dorsal root ganglia (DRG) metabolic characteristics at different time points post sciatic nerve injury and acquired hundreds of differentially changed metabolites. In addition, the results reveal that several metabolic pathways were significantly altered, such as 'Histidine metabolism', 'Glycine serine and threonine metabolism', 'Arginine and proline metabolism', 'taurine and hypotaurine metabolism' and so on. Given metabolite could alter a cell's or an organism's phenotype, further investigation demonstrated that N, N-dimethylglycine (DMG) has a promoting effect on the regenerative ability post injury. Overall, our data may serve as a resource useful for further understanding how metabolites contribute to axon regeneration in DRG during sciatic nerve regeneration and suggest DMG may be a candidate drug to repair nerve injury. Open Research DATA AVAILABILITY STATEMENT The metabolomic profiling data are made available in Datasets S1–S2. The other data sets generated during the current study are available from the corresponding author on reasonable request. Supporting Information Filename Description fsb222305-sup-0001-Supinfo.docxWord 2007 document , 1.4 MB Supplementary Material fsb222305-sup-0002-DatasetS1.xlsxExcel 2007 spreadsheet , 1,007.1 KB Dataset S1 fsb222305-sup-0003-DatasetS2.xlsxExcel 2007 spreadsheet , 899 KB Dataset S2 fsb222305-sup-0004-DatasetS3.xlsxExcel 2007 spreadsheet , 36.3 KB Dataset S3 fsb222305-sup-0005-DatasetS4.xlsxExcel 2007 spreadsheet , 12.9 KB Dataset S4 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume36, Issue5May 2022e22305 RelatedInformation
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